Detailed information of TA system TA146866

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 624569..626157
• Replicon: chromosome
• Accession: NZ_CP047600
• Organism: Escherichia coli O84:H7 strain Trh52

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: B7URP4
• Locus tag: GUK18_RS03045
• Protein ID: WP_001125430.1
• Coordinates: 624569..625891 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GUK18_RS03050
• Protein ID: WP_001334382.1
• Coordinates: 625891..626157 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUK18_RS03030	621963..622673	-	711	WP_001195181.1	fimbria/pilus periplasmic chaperone	-
GUK18_RS03035	623035..623598	-	564	WP_000768280.1	type 1 fimbrial major subunit FimA	-
GUK18_RS03040	624031..624297	-	267	Protein_600	helix-turn-helix transcriptional regulator	-
GUK18_RS03045	624569..625891	-	1323	WP_001125430.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
GUK18_RS03050	625891..626157	-	267	WP_001334382.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
GUK18_RS03055	626465..627223	+	759	WP_001286540.1	trans-aconitate 2-methyltransferase	-
GUK18_RS03060	627227..628141	-	915	WP_001313828.1	bestrophin family inner membrane protein	-
GUK18_RS03065	628338..629789	-	1452	WP_000854637.1	tagaturonate reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49297.96 Da
• Isoelectric Point: 8.1297

>T146866 WP_001125430.1 NZ_CP047600:c625891-624569 [Escherichia coli O84:H7]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTCPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGRVRALAVERFDRRW
NTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIAQIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T146866 NZ_CP062815:910353-910760 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10017.42 Da
• Isoelectric Point: 4.9632

>AT146866 WP_001334382.1 NZ_CP047600:c626157-625891 [Escherichia coli O84:H7]
MMSFQKIYSPMQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTSLTTFFKILQSLELSMTLCDAKNASPEA
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT146866 NZ_CP062815:910106-910372 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• AlphaFold DB: A0A0K4M2E2