Detailed information of TA system TA146839

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mqsRA (relBE)/MqsR-MqsA
• Location: 3764852..3765545
• Replicon: chromosome
• Accession: NZ_CP047594
• Organism: Escherichia coli strain LD27-1

Toxin (Protein)

• Gene name: mqsR
• Uniprot ID: S1EZG2
• Locus tag: GUU88_RS18250
• Protein ID: WP_000415584.1
• Coordinates: 3765249..3765545 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: mqsA
• Uniprot ID: S1EBV2
• Locus tag: GUU88_RS18245
• Protein ID: WP_000650107.1
• Coordinates: 3764852..3765247 (-)
• Length: 132 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU88_RS18235	3760716..3762974	-	2259	WP_001281881.1	DNA topoisomerase IV subunit A	-
GUU88_RS18240	3763112..3764719	-	1608	WP_001295629.1	ABC transporter substrate-binding protein	-
GUU88_RS18245	3764852..3765247	-	396	WP_000650107.1	type II toxin-antitoxin system antitoxin MqsA	Antitoxin
GUU88_RS18250	3765249..3765545	-	297	WP_000415584.1	type II toxin-antitoxin system toxin MqsR	Toxin
GUU88_RS18255	3765750..3766232	-	483	WP_053266176.1	GyrI-like domain-containing protein	-
GUU88_RS18260	3766285..3766677	-	393	WP_000712658.1	OB fold stress tolerance protein YgiW	-
GUU88_RS18265	3766829..3767488	+	660	WP_160194483.1	two-component system response regulator QseB	-
GUU88_RS18270	3767485..3768834	+	1350	WP_072656973.1	two-component system sensor histidine kinase QseC	-
GUU88_RS18275	3768880..3769212	-	333	WP_000917684.1	DUF2645 family protein	-
GUU88_RS18280	3769531..3770112	+	582	WP_000065430.1	NADPH:quinone oxidoreductase MdaB	-
GUU88_RS18285	3770143..3770457	+	315	WP_000958598.1	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin MqsR (5-97)

Antitoxin

• Zinc finger, MqsA-type (3-47)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11231.96 Da
• Isoelectric Point: 8.9070

>T146839 WP_000415584.1 NZ_CP047594:c3765545-3765249 [Escherichia coli]
MEKRTPHTRLSQVKKLVNAGQVRTTRSALLNADELGLDFDGMCNVIIGLSESDFYKSMTTYSDHTIWQDVYRPRLVTGQV
YLKITVIHDVLIVSFKEK

Nucleotide

• Length: 297 bp

>T146839 NZ_CP062811:c647149-646685 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAACCCATTAA

Antitoxin

Protein

• Length: 132 a.a.
• Molecular weight: 14703.10 Da
• Isoelectric Point: 9.2136

>AT146839 WP_000650107.1 NZ_CP047594:c3765247-3764852 [Escherichia coli]
MKCPVCHQGEMVSGIKDIPYTFRGRKTVLKGIHGLYCVHCEESIMNKEESDAFMAQVKAFRASVNAETVAPEFIVKVRKK
LSLTQKEASEIFGGGVNAFSRYEKGNAQPHPSTIKLLRVLDKHPELLNEIR

Nucleotide

• Length: 396 bp

>AT146839 NZ_CP062811:c647484-647149 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCTTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACGTTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 3HI2

Antitoxin

• PDB: 3HI2
• PDB: 3GA8
• PDB: 3O9X
• PDB: 2KZ8
• PDB: 3FMY
• PDB: 3GN5
• AlphaFold DB: A0A7U9LU65