Detailed information of TA system TA146838

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 3636117..3636771
• Replicon: chromosome
• Accession: NZ_CP047594
• Organism: Escherichia coli strain LD27-1

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: GUU88_RS17605
• Protein ID: WP_000244777.1
• Coordinates: 3636117..3636524 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: GUU88_RS17610
• Protein ID: WP_029392880.1
• Coordinates: 3636505..3636771 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU88_RS17585	3632074..3633807	-	1734	WP_000813200.1	single-stranded-DNA-specific exonuclease RecJ	-
GUU88_RS17590	3633813..3634523	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
GUU88_RS17595	3634548..3635444	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
GUU88_RS17600	3635556..3636077	+	522	WP_001055874.1	flavodoxin FldB	-
GUU88_RS17605	3636117..3636524	-	408	WP_000244777.1	toxin CptA	Toxin
GUU88_RS17610	3636505..3636771	-	267	WP_029392880.1	FAD assembly factor SdhE	Antitoxin
GUU88_RS17615	3637014..3637994	+	981	WP_000886062.1	tRNA-modifying protein YgfZ	-
GUU88_RS17620	3638190..3638849	-	660	WP_000250274.1	hemolysin III family protein	-
GUU88_RS17625	3639013..3639324	-	312	WP_001182957.1	N(4)-acetylcytidine aminohydrolase	-
GUU88_RS17630	3639369..3640802	+	1434	WP_001336277.1	6-phospho-beta-glucosidase BglA	-
GUU88_RS17635	3640859..3641602	-	744	WP_000951964.1	SDR family oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T146838 WP_000244777.1 NZ_CP047594:c3636524-3636117 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T146838 NZ_CP062811:c393723-393238 [Escherichia coli]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCATTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTTATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCTCATTACTCTG
GAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10520.10 Da
• Isoelectric Point: 5.1599

>AT146838 WP_029392880.1 NZ_CP047594:c3636771-3636505 [Escherichia coli]
MDINSKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT146838 NZ_CP062811:c393992-393714 [Escherichia coli]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTGGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7