Detailed information of TA system TA146783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): rnlAB/RnlA-RnlB
• Location: 3996221..3997646
• Replicon: chromosome
• Accession: NZ_CP047576
• Organism: Escherichia coli strain 94EC

Toxin (Protein)

• Gene name: rnlA
• Uniprot ID: -
• Locus tag: GUJ28_RS19175
• Protein ID: WP_024190590.1
• Coordinates: 3996221..3997291 (+)
• Length: 357 a.a.

Antitoxin (Protein)

• Gene name: rnlB
• Uniprot ID: E6BSF0
• Locus tag: GUJ28_RS19180
• Protein ID: WP_000461705.1
• Coordinates: 3997284..3997646 (+)
• Length: 121 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUJ28_RS19160	3991838..3995236	+	3399	WP_071938245.1	prophage tail fiber N-terminal domain-containing protein	-
GUJ28_RS19165	3995236..3995820	+	585	WP_063112476.1	tail fiber assembly protein	-
GUJ28_RS19170	3996006..3996161	+	156	WP_001597763.1	hypothetical protein	-
GUJ28_RS19175	3996221..3997291	+	1071	WP_024190590.1	type II toxin-antitoxin system RnlA family toxin	Toxin
GUJ28_RS19180	3997284..3997646	+	363	WP_000461705.1	type II toxin-antitoxin system RnlB family antitoxin	Antitoxin
GUJ28_RS19185	3997784..3998053	-	270	WP_000429347.1	hypothetical protein	-
GUJ28_RS19195	3998931..3999413	-	483	WP_000162574.1	SsrA-binding protein SmpB	-
GUJ28_RS19200	3999584..4000021	+	438	WP_001341638.1	type II toxin-antitoxin system toxin RatA	-
GUJ28_RS19205	4000011..4000301	+	291	WP_001117838.1	RnfH family protein	-
GUJ28_RS19210	4000363..4000704	-	342	WP_001203437.1	outer membrane protein assembly factor BamE	-
GUJ28_RS19215	4000853..4002514	-	1662	WP_000880910.1	DNA repair protein RecN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Bacterial toxin, RNase RnlA/LsoA, N-terminal repeated domain (98-183)

Antitoxin

• Antitoxin RnlB/LsoB (15-112)

Sequences

Toxin

Protein

• Length: 357 a.a.
• Molecular weight: 40475.40 Da
• Isoelectric Point: 7.2332

>T146783 WP_024190590.1 NZ_CP047576:3996221-3997291 [Escherichia coli]
MTVRNYTNLNLDRTTIEATSRAFIENKNYSVHSIGPMPGARAGLRVVFAKPGEALATVNIFYNNGGTSTVQYQTGANRNL
GKELADNLYETINPAEFEQVNMVLQGFVEANVLSVLQLSAEQPHIQFYEYLRNTHTTVWKIHSPEFQDELTVSLHHRNGT
LQIQGRPLSCYRVFIFNLSELLDLQGLEKVLIRQDDGKAYIVQKEVARSHLECEMGDAYPLLHKNVEKLLVSGLCVKLAA
PDLPDYCMLLYPELRSVEGVLKSTMYRYGMPITQDGFGPYFDKIGSEFILKQQFGRNLSSAKVKTINDAYTFFNRERHGL
FHMEIVVDTSRMVSDMARLMTKARQAWGIIKDLYIV

Nucleotide

• Length: 1071 bp

>T146783 NZ_CP062782:751357-751671 [Escherichia coli O157:H7]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTAGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTTTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 121 a.a.
• Molecular weight: 13304.23 Da
• Isoelectric Point: 4.2386

>AT146783 WP_000461705.1 NZ_CP047576:3997284-3997646 [Escherichia coli]
MFEITGVNVSGALIAVVMATGYENPLTSVHEIEKRLSVILGNSQATGEVLFDLLCSNGPEWNRFVSLEIRCGSILLDTAK
IVDEQEIPAQILRHQRDVLRNHPEYVEDSVLTPDDVLCIL

Nucleotide

• Length: 363 bp

>AT146783 NZ_CP062782:751668-752084 [Escherichia coli O157:H7]
ATGATTGCGATTGCCGACATCTTGCATGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACATCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Antitoxin

• AlphaFold DB: A0A1X9TDS3