Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 3001544..3001801 Replicon chromosome
Accession NZ_CP047576
Organism Escherichia coli strain 94EC

Toxin (Protein)


Gene name hokA Uniprot ID E9T8R9
Locus tag GUJ28_RS14275 Protein ID WP_001135746.1
Coordinates 3001649..3001801 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name sokA
Locus tag -
Coordinates 3001544..3001598 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GUJ28_RS14255 2996769..2997764 - 996 WP_001182653.1 O-acetyltransferase WecH -
GUJ28_RS14260 2997939..2998238 + 300 WP_000980111.1 YsaB family lipoprotein -
GUJ28_RS14265 2998333..2999244 + 912 WP_001168544.1 glycine--tRNA ligase subunit alpha -
GUJ28_RS14270 2999254..3001323 + 2070 WP_001291774.1 glycine--tRNA ligase subunit beta -
- 3001544..3001598 - 55 - - Antitoxin
GUJ28_RS14275 3001649..3001801 + 153 WP_001135746.1 type I toxin-antitoxin system toxin HokA Toxin
GUJ28_RS14280 3001989..3002201 - 213 WP_000014594.1 RNA chaperone/antiterminator CspA -
GUJ28_RS14285 3002482..3002772 - 291 WP_000455798.1 HTH-type transcriptional regulator -
GUJ28_RS14290 3003206..3003916 + 711 WP_000190516.1 DUF3053 domain-containing protein -
GUJ28_RS14295 3003966..3004940 - 975 WP_000805027.1 glyoxylate/hydroxypyruvate reductase GhrB -
GUJ28_RS14300 3005044..3005703 - 660 WP_000747625.1 OmpA family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(5-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5928.14 Da        Isoelectric Point: 7.7146

>T146777 WP_001135746.1 NZ_CP047576:3001649-3001801 [Escherichia coli]
MPQKYRLLSLIVICYTLLFFTWMIRDSLCELHIKQGSYELAAFLACNLKE

Download         Length: 153 bp

>T146777 NZ_CP062782:283379-283486 [Escherichia coli O157:H7]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 55 bp

>AT146777 NZ_CP047576:c3001598-3001544 [Escherichia coli]
GTTCAGGCATAGGGTAGCCTCACTTTGATTTATAGTCAGGTGGGGCTTTTCTCTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H0JN89

Antitoxin

Download structure file

References