Detailed information of TA system TA146192

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 110225..110784
• Replicon: plasmid pMS6193A-NDM
• Accession: NZ_CP047406
• Organism: Escherichia coli strain MS6193

Toxin (Protein)

• Gene name: relE
• Uniprot ID: V0SVC8
• Locus tag: MS6193_RS24480
• Protein ID: WP_000421272.1
• Coordinates: 110509..110784 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SRR3
• Locus tag: MS6193_RS24475
• Protein ID: WP_001387467.1
• Coordinates: 110225..110509 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
MS6193_RS24445	105688..106128	-	441	Protein_133	zinc-binding dehydrogenase	-
MS6193_RS24450	106102..106296	+	195	WP_000796505.1	hypothetical protein	-
MS6193_RS24455	106529..107419	+	891	WP_001077068.1	dihydrodipicolinate synthase family protein	-
MS6193_RS24460	107444..107824	+	381	WP_000710783.1	Rid family detoxifying hydrolase	-
MS6193_RS24465	107857..108822	+	966	WP_001022265.1	2-keto-3-deoxygluconate transporter	-
MS6193_RS24470	108868..109620	-	753	WP_000562172.1	IclR family transcriptional regulator	-
MS6193_RS24475	110225..110509	+	285	WP_001387467.1	ribbon-helix-helix domain-containing protein	Antitoxin
MS6193_RS24480	110509..110784	+	276	WP_000421272.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
MS6193_RS24485	110890..111183	+	294	WP_001105060.1	hypothetical protein	-
MS6193_RS24490	111218..111346	-	129	Protein_142	IS481 family transposase	-
MS6193_RS24495	111379..112548	-	1170	WP_001072355.1	hypothetical protein	-
MS6193_RS24500	113041..113348	+	308	Protein_144	type II toxin-antitoxin system ParD family antitoxin	-
MS6193_RS24505	113691..113873	+	183	WP_042065278.1	hypothetical protein	-
MS6193_RS24510	113994..114734	+	741	WP_001066942.1	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaNDM-1 / sul1 / qacE / aadA2 / dfrA12	-	1..142890	142890

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10749.45 Da
• Isoelectric Point: 10.0871

>T146192 WP_000421272.1 NZ_CP047406:110509-110784 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T146192 NZ_CP062729:1093268-1093603 [Escherichia coli O157:H7]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCCGG
ACATCGTCCGGCTGTTGTCCTGAGTCCGTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACGGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10460.87 Da
• Isoelectric Point: 5.6558

>AT146192 WP_001387467.1 NZ_CP047406:110225-110509 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT146192 NZ_CP062729:1093020-1093268 [Escherichia coli O157:H7]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: V0SVC8

Antitoxin

• AlphaFold DB: A0A0E0Y880