146153

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yafW-ykfI/CbtA-CbeA
Location 312357..313036 Replicon chromosome
Accession NZ_CP047405
Organism Escherichia coli strain MS6193

Toxin (Protein)


Gene name ykfI Uniprot ID A0A2I8S2T2
Locus tag MS6193_RS01465 Protein ID WP_008324514.1
Coordinates 312357..312698 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name yafW Uniprot ID A0A2I8S2U6
Locus tag MS6193_RS01470 Protein ID WP_008324516.1
Coordinates 312719..313036 (-) Length 106 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6193_RS01440 307634..308035 + 402 WP_000174677.1 sigma factor-binding protein Crl -
MS6193_RS01445 308074..309129 - 1056 WP_000749881.1 phosphoporin PhoE -
MS6193_RS01450 309417..310520 + 1104 WP_001285288.1 glutamate 5-kinase -
MS6193_RS01455 310532..311785 + 1254 WP_000893255.1 glutamate-5-semialdehyde dehydrogenase -
MS6193_RS01465 312357..312698 - 342 WP_008324514.1 type IV toxin-antitoxin system toxin YkfI Toxin
MS6193_RS01470 312719..313036 - 318 WP_008324516.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
MS6193_RS01475 313049..313276 - 228 WP_008324517.1 DUF987 domain-containing protein -
MS6193_RS01480 313285..313761 - 477 WP_000811693.1 RadC family protein -
MS6193_RS01485 313777..314235 - 459 WP_000211838.1 antirestriction protein -
MS6193_RS01490 314333..314572 - 240 WP_000194654.1 DUF905 domain-containing protein -
MS6193_RS01495 314649..315116 - 468 WP_001547765.1 hypothetical protein -
MS6193_RS01500 315139..315582 - 444 WP_008324520.1 hypothetical protein -
MS6193_RS01505 315582..315818 - 237 WP_001115854.1 hypothetical protein -
MS6193_RS01510 315855..316556 - 702 WP_008324530.1 WYL domain-containing protein -
MS6193_RS01515 316774..317595 - 822 WP_001548934.1 DUF945 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 312357..322004 9647


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(6-104)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12919.98 Da        Isoelectric Point: 9.6543

>T146153 WP_008324514.1 NZ_CP047405:c312698-312357 [Escherichia coli]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Download         Length: 342 bp

>T146153 NZ_CP062726:51521-51826 [Escherichia coli O157:H7]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 106 a.a.        Molecular weight: 11787.33 Da        Isoelectric Point: 7.3476

>AT146153 WP_008324516.1 NZ_CP047405:c313036-312719 [Escherichia coli]
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Download         Length: 318 bp

>AT146153 NZ_CP062726:51301-51519 [Escherichia coli O157:H7]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2I8S2T2

Antitoxin

Source ID Structure
AlphaFold DB A0A2I8S2U6

References