Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 620532..621151 Replicon chromosome
Accession NZ_CP047336
Organism Klebsiella pneumoniae strain 2019036D

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GTH21_RS03070 Protein ID WP_002892050.1
Coordinates 620532..620750 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag GTH21_RS03075 Protein ID WP_002892066.1
Coordinates 620777..621151 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GTH21_RS03035 616579..616842 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
GTH21_RS03040 616842..616982 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
GTH21_RS03045 616979..617677 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
GTH21_RS03050 617778..619229 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
GTH21_RS03055 619204..619674 - 471 WP_002892026.1 YlaC family protein -
GTH21_RS03060 619695..619835 + 141 WP_004147370.1 hypothetical protein -
GTH21_RS03065 619807..620373 - 567 WP_002892030.1 maltose O-acetyltransferase -
GTH21_RS03070 620532..620750 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GTH21_RS03075 620777..621151 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
GTH21_RS03080 621637..624783 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
GTH21_RS03085 624806..625999 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T145986 WP_002892050.1 NZ_CP047336:c620750-620532 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T145986 NZ_CP062715:c457209-456724 [Escherichia coli O157:H7]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT145986 WP_002892066.1 NZ_CP047336:c621151-620777 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT145986 NZ_CP062715:c457478-457200 [Escherichia coli O157:H7]
ATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGCAGCGAA
TCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATCAACGAC
TATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAGGCCAAA
ATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References