Detailed information of TA system TA145576

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 35845..36322
• Replicon: plasmid unnamed1
• Accession: NZ_CP047219
• Organism: Sphingobium yanoikuyae strain YC-JY1

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: GS397_RS26265
• Protein ID: WP_159368165.1
• Coordinates: 35845..36111 (-)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: -
• Locus tag: GS397_RS26270
• Protein ID: WP_159368166.1
• Coordinates: 36095..36322 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS397_RS26240	31543..32892	-	1350	WP_159368160.1	site-specific integrase	-
GS397_RS26245	33017..33304	-	288	WP_159368161.1	hypothetical protein	-
GS397_RS26250	33301..34458	-	1158	WP_159368162.1	phosphoadenosine phosphosulfate reductase family protein	-
GS397_RS26255	34463..35242	-	780	WP_159368163.1	hypothetical protein	-
GS397_RS26260	35261..35767	-	507	WP_159368164.1	hypothetical protein	-
GS397_RS26265	35845..36111	-	267	WP_159368165.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GS397_RS26270	36095..36322	-	228	WP_159368166.1	ribbon-helix-helix protein, CopG family	Antitoxin
GS397_RS28120	36378..36887	-	510	WP_201450355.1	hypothetical protein	-
GS397_RS26280	36904..37203	-	300	WP_159368167.1	hypothetical protein	-
GS397_RS26285	37215..37886	-	672	WP_159368168.1	hypothetical protein	-
GS397_RS26290	37973..38410	-	438	WP_159368169.1	hypothetical protein	-
GS397_RS26295	38770..40236	+	1467	WP_159368170.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..135293	135293
	-	flank	IS/Tn	-	-	40775..41650	875

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10018.55 Da
• Isoelectric Point: 10.8652

>T145576 WP_159368165.1 NZ_CP047219:c36111-35845 [Sphingobium yanoikuyae]
MAWQIELSSSAEKSLSKLDRQTAKRIITFLRERVASSDDPRASGKALTGPLAGRWRYRVGDYRIVCEIEDRRLVVLVLTV
GHRSGVYR

Nucleotide

• Length: 267 bp

>T145576 NZ_CP062407:c863836-863660 [Staphylococcus aureus]
TTGGATAGATGGTGGCTATCTGAGTATAAGGAGGTGGTGCCTATGGTGGCATTACTGAAATCTTTAGAAAGGAGACGCCT
AATGATTACAATTAGTACCATGTTGCAGTTTGGTTTATTCCTTATTGCATTGATAGGTCTAGTAATCAAGCTTATTGAAT
TAAGCAATAAAAAATAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8524.58 Da
• Isoelectric Point: 4.4179

>AT145576 WP_159368166.1 NZ_CP047219:c36322-36095 [Sphingobium yanoikuyae]
MATSVRLAPEIEQRLDHLASATGRTKAYYLREIIERGLEDMEDIYLSDKVLEDIRAGRETTSSLEDVEKRLGLAD

Nucleotide

• Length: 228 bp

>AT145576 NZ_CP062407:863537-863592 [Staphylococcus aureus]
AAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTG