Detailed information of TA system TA145557

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 68344..69071
• Replicon: plasmid unnamed1
• Accession: NZ_CP047193
• Organism: Klebsiella pneumoniae strain Kp36

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7W3D9W1
• Locus tag: GS419_RS27305
• Protein ID: WP_011251285.1
• Coordinates: 68344..68655 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GS419_RS27310
• Protein ID: WP_011251286.1
• Coordinates: 68652..69071 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GS419_RS27265	63355..63865	+	511	Protein_72	hypothetical protein	-
GS419_RS27270	63855..64295	+	441	WP_011251275.1	hypothetical protein	-
GS419_RS27275	64720..65676	-	957	WP_011251280.1	DsbA family protein	-
GS419_RS27280	65736..66077	-	342	WP_011251281.1	hypothetical protein	-
GS419_RS27285	66091..66402	-	312	WP_011251282.1	hypothetical protein	-
GS419_RS27290	66419..66853	-	435	Protein_77	thioredoxin fold domain-containing protein	-
GS419_RS27295	66870..67567	-	698	Protein_78	IS1 family transposase	-
GS419_RS27300	67681..68139	+	459	Protein_79	DDE-type integrase/transposase/recombinase	-
GS419_RS27305	68344..68655	+	312	WP_011251285.1	type II toxin-antitoxin system HigB family toxin	Toxin
GS419_RS27310	68652..69071	+	420	WP_011251286.1	helix-turn-helix domain-containing protein	Antitoxin
GS419_RS27315	69218..70186	+	969	WP_074428168.1	IS5 family transposase	-
GS419_RS27320	70288..70623	-	336	Protein_83	conjugal transfer protein TraI	-
GS419_RS27325	70637..71425	-	789	WP_040217257.1	hypothetical protein	-
GS419_RS27330	71446..71889	-	444	WP_159351770.1	hypothetical protein	-
GS419_RS30195	72485..73240	+	756	Protein_86	hypothetical protein	-
GS419_RS27340	73433..73903	+	471	WP_048333570.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	rmpA / iroN / rmpA2 / iutA / iucD / iucC / iucB / iucA	1..219800	219800
	-	inside	IScluster/Tn	-	-	61435..85742	24307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (22-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (85-138)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12346.12 Da
• Isoelectric Point: 9.7248

>T145557 WP_011251285.1 NZ_CP047193:68344-68655 [Klebsiella pneumoniae]
MHVISKEPFEEAAKRYPNDSLAIRALYRLVRETDFSSPAEMLTLIPSLDNFKYRNKWWVLDVGGNNLRVIAYINFVNKRF
YVKHIATHADYDKLTRYYRENKE

Nucleotide

• Length: 312 bp

>T145557 NZ_CP062405:c607147-606959 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15377.46 Da
• Isoelectric Point: 4.4420

>AT145557 WP_011251286.1 NZ_CP047193:68652-69071 [Klebsiella pneumoniae]
MITDTAKAIEATKQLVAAVPFLGGSSSESDYREAMELVDYLIENDDENPLIDFLASKIADYEDNSPRFAEFNKAIAEIPV
GVALLRTLIDQHKLSYSDLKDEIGSKSLVSQILSGQRSLTITHIKALSARFGVKPEWFL

Nucleotide

• Length: 420 bp

>AT145557 NZ_CP062405:c607436-607176 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A7W3D9W1