Detailed information of TA system TA145535

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1392749..1393226
• Replicon: chromosome
• Accession: NZ_CP047171
• Organism: Xylella fastidiosa subsp. fastidiosa strain IVIA5235

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: Q87CA4
• Locus tag: D1605_RS06295
• Protein ID: WP_011097988.1
• Coordinates: 1392960..1393226 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: A0A7G7TPD2
• Locus tag: D1605_RS06290
• Protein ID: WP_004091377.1
• Coordinates: 1392749..1392976 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D1605_RS06250	1387876..1389021	-	1146	WP_011097984.1	baseplate J/gp47 family protein	-
D1605_RS06255	1389120..1389416	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D1605_RS06260	1389420..1389725	+	306	WP_004089254.1	putative addiction module antidote protein	-
D1605_RS06265	1389736..1390089	-	354	WP_011097986.1	hypothetical protein	-
D1605_RS06270	1390086..1390727	-	642	WP_187120434.1	hypothetical protein	-
D1605_RS06275	1390724..1391554	-	831	WP_004090766.1	hypothetical protein	-
D1605_RS06280	1391551..1391868	-	318	WP_011097873.1	hypothetical protein	-
D1605_RS06285	1391868..1392638	-	771	WP_004090769.1	hypothetical protein	-
D1605_RS06290	1392749..1392976	+	228	WP_004091377.1	hypothetical protein	Antitoxin
D1605_RS06295	1392960..1393226	+	267	WP_011097988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D1605_RS06300	1393237..1395126	-	1890	WP_011097989.1	hypothetical protein	-
D1605_RS06305	1395083..1395247	-	165	WP_014607750.1	hypothetical protein	-
D1605_RS06310	1395274..1395696	-	423	WP_004085005.1	hypothetical protein	-
D1605_RS06315	1395693..1396130	-	438	WP_011097990.1	DUF3277 family protein	-
D1605_RS06320	1396140..1396361	-	222	Protein_1227	DUF3383 family protein	-
D1605_RS06325	1396390..1397595	-	1206	Protein_1228	transposase	-
D1605_RS06330	1397589..1398194	-	606	Protein_1229	IS607 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1386071..1404370	18299
	-	flank	IS/Tn	-	-	1397589..1397810	221

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-87)

Antitoxin

• Protein of unknown function DUF6290 (1-71)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10163.79 Da
• Isoelectric Point: 10.6086

>T145535 WP_011097988.1 NZ_CP047171:1392960-1393226 [Xylella fastidiosa subsp. fastidiosa]
MAWTIDYTDTAKQQLRKLDKHMARRIVDFMDERIAGLENPRSSGKALTGPLGGFWRYRVGDFRVVCAIQDSVLRVLVVRV
GHRGEIYR

Nucleotide

• Length: 267 bp

>T145535 NZ_CP062402:c2421187-2420921 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCGGCGAAGTTAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAC
ATTTATAGAAATTGTTGAGACTTTAAAAAATAATCCTTATAAAATAACACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCTTCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8615.63 Da
• Isoelectric Point: 5.0958

>AT145535 WP_004091377.1 NZ_CP047171:1392749-1392976 [Xylella fastidiosa subsp. fastidiosa]
MATSIRLSPEMEQRLNSLASHTGRTKAYYLREIIEHGIEEMEDYYLAADVLERVRHGQEQVHSAADVRKTLGLDD

Nucleotide

• Length: 228 bp

>AT145535 NZ_CP062402:c2421444-2421187 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGTAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATGT
ATCTTGTATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAATAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: A0A060HDH1

Antitoxin

• AlphaFold DB: A0A7G7TPD2