Detailed information of TA system TA145312

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 3705308..3705906
• Replicon: chromosome
• Accession: NZ_CP047010
• Organism: Escherichia coli strain 19-5

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: GQF95_RS17975
• Protein ID: WP_053291281.1
• Coordinates: 3705308..3705685 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: GQF95_RS17980
• Protein ID: WP_001603498.1
• Coordinates: 3705685..3705906 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GQF95_RS25810 (3702213)	3702213..3702296	-	84	WP_211180506.1	protein YqgH	-
GQF95_RS17955 (3702449)	3702449..3703156	+	708	WP_000234517.1	DUF554 domain-containing protein	-
GQF95_RS17965 (3703535)	3703535..3704797	+	1263	WP_113402210.1	integrase arm-type DNA-binding domain-containing protein	-
GQF95_RS17970 (3704983)	3704983..3705198	+	216	Protein_3533	transposase	-
GQF95_RS17975 (3705308)	3705308..3705685	-	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GQF95_RS17980 (3705685)	3705685..3705906	-	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GQF95_RS17985 (3706091)	3706091..3707110	-	1020	WP_095784823.1	fimbrial protein	-
GQF95_RS17990 (3707122)	3707122..3709608	-	2487	WP_096218945.1	fimbrial biogenesis outer membrane usher protein	-
GQF95_RS17995 (3709626)	3709626..3710306	-	681	WP_053270475.1	fimbrial assembly chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3704983..3718331	13348
	-	flank	IS/Tn	-	-	3704983..3705264	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T145312 WP_053291281.1 NZ_CP047010:c3705685-3705308 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T145312 NZ_CP062354:2639870-2640079 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT145312 WP_001603498.1 NZ_CP047010:c3705906-3705685 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT145312 NZ_CP062354:2639565-2639858 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGATAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: L4J1C0