Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3633312..3633966 Replicon chromosome
Accession NZ_CP047010
Organism Escherichia coli strain 19-5

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag GQF95_RS17600 Protein ID WP_000244777.1
Coordinates 3633312..3633719 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GQF95_RS17605 Protein ID WP_000354046.1
Coordinates 3633700..3633966 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GQF95_RS17580 (3629269) 3629269..3631002 - 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -
GQF95_RS17585 (3631008) 3631008..3631718 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GQF95_RS17590 (3631743) 3631743..3632639 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GQF95_RS17595 (3632751) 3632751..3633272 + 522 WP_001055874.1 flavodoxin FldB -
GQF95_RS17600 (3633312) 3633312..3633719 - 408 WP_000244777.1 protein YgfX Toxin
GQF95_RS17605 (3633700) 3633700..3633966 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GQF95_RS17610 (3634209) 3634209..3635189 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
GQF95_RS17615 (3635385) 3635385..3636044 - 660 WP_000250274.1 hemolysin III family protein -
GQF95_RS17620 (3636208) 3636208..3636519 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
GQF95_RS17625 (3636564) 3636564..3637997 + 1434 WP_063502079.1 6-phospho-beta-glucosidase BglA -
GQF95_RS17630 (3638054) 3638054..3638797 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T145311 WP_000244777.1 NZ_CP047010:c3633719-3633312 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T145311 NZ_CP062354:2582609-2582713 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGGTTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT145311 WP_000354046.1 NZ_CP047010:c3633966-3633700 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT145311 NZ_CP062354:c2582871-2582708 [Staphylococcus aureus]
GTAGTAAGTAGAAGCAAAAGATGAAAATCTTTAACTCTTGAAACACAAAAAGGGCAACACTCGGAAACATGTTACCCTAA
TGAGCCCGTTAAAAAGACGGTGACCTTATATTTTATTTAAAAATAGCCTTCAAAATGCCGGTCAAAGCGAATAGAAGGTT
ATTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References