Detailed information of TA system TA144849

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2281239..2283064
• Replicon: chromosome
• Accession: NZ_CP046740
• Organism: Vibrio cholerae strain 3566-06

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: GPW70_RS15325
• Protein ID: WP_032472659.1
• Coordinates: 2281239..2282600 (+)
• Length: 454 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: GPW70_RS15330
• Protein ID: WP_032472658.1
• Coordinates: 2282597..2283064 (+)
• Length: 156 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPW70_RS15290	2276273..2276701	+	429	WP_019830040.1	DUF2787 domain-containing protein	-
GPW70_RS15295	2276752..2277198	+	447	WP_019830041.1	DUF2787 domain-containing protein	-
GPW70_RS15300	2277301..2277828	+	528	WP_019830042.1	Flp pilus assembly complex ATPase component TadA	-
GPW70_RS15305	2277928..2278287	+	360	WP_134983340.1	hypothetical protein	-
GPW70_RS15310	2278320..2278961	-	642	WP_032472660.1	inovirus Gp2 family protein	-
GPW70_RS15315	2280421..2280621	-	201	WP_019830047.1	AlpA family transcriptional regulator	-
GPW70_RS15325	2281239..2282600	+	1362	WP_032472659.1	type II toxin-antitoxin system HipA family toxin	Toxin
GPW70_RS15330	2282597..2283064	+	468	WP_032472658.1	helix-turn-helix domain-containing protein	Antitoxin
GPW70_RS15335	2283492..2284070	+	579	WP_000109982.1	class I SAM-dependent methyltransferase	-
GPW70_RS15340	2284074..2287010	+	2937	WP_032472657.1	DEAD/DEAH box helicase family protein	-
GPW70_RS15345	2287081..2288030	+	950	Protein_2005	NERD domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	acfD / acfA / acfC / acfB / tcpJ / tcpN/toxT / tcpF / tcpE / tcpT / tcpS / tcpD / tcpR / tcpC / tcpQ / tcpB / tcpA / tcpH / tcpP / tcpI	2237767..2288628	50861

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (166-383)

Antitoxin

• Cro/C1-type helix-turn-helix domain (80-138)

Sequences

Toxin

Protein

• Length: 454 a.a.
• Molecular weight: 51377.35 Da
• Isoelectric Point: 5.0463

>T144849 WP_032472659.1 NZ_CP046740:2281239-2282600 [Vibrio cholerae]
MEELTVQAFIRGQWIDIGIISFPKSSQHNFRVTELNYLSDYALKHYDKDDFHAVSLNHPVSFFFDDMGKPGWLKFLDDIM
PSGASRRYWVKHLDIEDLSSDEQDYVLLKFGTMSPIGNLRIKDSLPERYEVADNLYFSVDDVKNRAGDFLDYAQQRGAAA
GGATGAGGEAPKLILRCGFDHGSGSEKIWIDPYQDDNSNHDLHYLVKYPRGSRSTIDCNILRAEFYFYHELTEMGVETIS
TDGMRLEEGLNYPSLWLPRFDVQIIDQQIERFGMESVYSILNKGAGVTLDHETTIRTLIEKITESNMVKHQGYRFDTQAF
VIEWVKRDLLNILFGNSDNHGRNTSFLKGDGVIKLAPIYDFAPMKADPAGIPRTTKWKAPLEVGGTYDFVGIADTLSDLV
PKEMLLKELAITASKCVGLKQRLALRGVPEQILEMPAVGLNYLSEKLTKWELL

Nucleotide

• Length: 1362 bp

>T144849 NZ_CP062243:c2904450-2904115 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 156 a.a.
• Molecular weight: 17073.60 Da
• Isoelectric Point: 10.7084

>AT144849 WP_032472658.1 NZ_CP046740:2282597-2283064 [Vibrio cholerae]
MKPSNKESVQDTVARLRANRNQQQPKVGKNATHSIKEQTPLDTPTRSKASKNAKAVSAAERKTEANKIIKHLLLGELTQG
QALKSLRINILGLKQDVFARLVDVSRKTLSDIENDRGSYNTEILNKVFKPFSLKIGLLPSSPDALKSLLMDGEDG

Nucleotide

• Length: 468 bp

>AT144849 NZ_CP062243:c2904698-2904450 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA