Detailed information of TA system TA144737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2418261..2418899
• Replicon: chromosome
• Accession: NZ_CP046716
• Organism: Escherichia coli strain T16R

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: GPL39_RS11605
• Protein ID: WP_000813794.1
• Coordinates: 2418723..2418899 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GPL39_RS11600
• Protein ID: WP_001270286.1
• Coordinates: 2418261..2418677 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GPL39_RS11580	2413413..2414354	-	942	WP_001251313.1	ABC transporter permease	-
GPL39_RS11585	2414355..2415368	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
GPL39_RS11590	2415386..2416531	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
GPL39_RS11595	2416776..2418182	-	1407	WP_000760585.1	PLP-dependent aminotransferase family protein	-
GPL39_RS11600	2418261..2418677	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GPL39_RS11605	2418723..2418899	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
GPL39_RS11610	2419121..2419351	+	231	WP_000494244.1	YncJ family protein	-
GPL39_RS11615	2419443..2421404	-	1962	WP_001301045.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GPL39_RS11620	2421477..2422013	-	537	WP_000429133.1	DNA-binding transcriptional regulator SutR	-
GPL39_RS11625	2422066..2423280	+	1215	WP_113402181.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2423320..2424585	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T144737 WP_000813794.1 NZ_CP046716:c2418899-2418723 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T144737 NZ_CP062239:c2082946-2082692 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT144737 WP_001270286.1 NZ_CP046716:c2418677-2418261 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT144737 NZ_CP062239:c2083194-2082943 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Toxin

• PDB: 6HPB