Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 401727..402373 Replicon chromosome
Accession NZ_CP046645
Organism Klebsiella pneumoniae strain EGKP3

Toxin (Protein)


Gene name higB Uniprot ID A0A483PC41
Locus tag GPU01_RS01920 Protein ID WP_004216295.1
Coordinates 401727..402074 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID W8UB68
Locus tag GPU01_RS01925 Protein ID WP_002920557.1
Coordinates 402074..402373 (+) Length 100 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPU01_RS01910 (GPU01_01875) 397653..399086 + 1434 WP_004889438.1 glycogen synthase GlgA -
GPU01_RS01915 (GPU01_01880) 399104..401551 + 2448 WP_002920561.1 glycogen phosphorylase -
GPU01_RS01920 (GPU01_01885) 401727..402074 + 348 WP_004216295.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GPU01_RS01925 (GPU01_01890) 402074..402373 + 300 WP_002920557.1 helix-turn-helix transcriptional regulator Antitoxin
GPU01_RS01930 (GPU01_01895) 402436..403944 - 1509 WP_002920554.1 glycerol-3-phosphate dehydrogenase -
GPU01_RS01935 (GPU01_01900) 404149..404478 + 330 WP_002920552.1 thiosulfate sulfurtransferase GlpE -
GPU01_RS01940 (GPU01_01905) 404529..405359 + 831 WP_004151408.1 rhomboid family intramembrane serine protease GlpG -
GPU01_RS01945 (GPU01_01910) 405409..406167 + 759 WP_002920548.1 DeoR/GlpR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(13-107)

Antitoxin

HigA2-like, helix-turn-helix domain

(9-87)


Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 13606.61 Da        Isoelectric Point: 5.6749

>T144636 WP_004216295.1 NZ_CP046645:401727-402074 [Klebsiella pneumoniae]
MWDVETTDTFDTWFELQSRALKEDMLATMLILSEFAPQLGRPYVDTVKDSTFQNMKELRVQHHGLPIRAFFAFDPLRKAI
VLCADNKDGMNEKRFYKEMITLADREFSQHLTKER

Download         Length: 348 bp

>T144636 NZ_CP062204:3949360-3949641 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAAGTTGCACAAAAAAAGCGTCATAAGGATAT
GAATAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGC
AAGGTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTA
CGATTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 100 a.a.        Molecular weight: 10800.58 Da        Isoelectric Point: 10.1464

>AT144636 WP_002920557.1 NZ_CP046645:402074-402373 [Klebsiella pneumoniae]
MATLKELMAKQSPDSQQRIAAKAAEIRQSVALNLLREELQMSQTEMAAAMGVKQPTIAKMEQADNDPRLSTLKRYIAALG
GELSINVTLPTGKKVSFNL

Download         Length: 300 bp

>AT144636 NZ_CP062204:3949098-3949379 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGAATTTAAATATGATTCAAAGGGATATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483PC41

Antitoxin

Source ID Structure
AlphaFold DB A0A0E1CBF8

References