Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/RelE-HTH
Location 2577280..2577969 Replicon chromosome
Accession NZ_CP046612
Organism Klebsiella pneumoniae strain WCGKP294

Toxin (Protein)


Gene name higB Uniprot ID A0A331C6E2
Locus tag GPJ73_RS12815 Protein ID WP_021469727.1
Coordinates 2577280..2577597 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A6B0N7G3
Locus tag GPJ73_RS12820 Protein ID WP_020804705.1
Coordinates 2577673..2577969 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GPJ73_RS12785 2572982..2573491 + 510 WP_020802144.1 GNAT family N-acetyltransferase -
GPJ73_RS12790 2573501..2574427 + 927 WP_032435210.1 amino acid ABC transporter permease -
GPJ73_RS12795 2574411..2575190 + 780 WP_002906697.1 amino acid ABC transporter ATP-binding protein -
GPJ73_RS12800 2575229..2576080 + 852 WP_072198177.1 transporter substrate-binding domain-containing protein -
GPJ73_RS12805 2576158..2576775 + 618 WP_032425015.1 glutathione S-transferase family protein -
GPJ73_RS12810 2576846..2577073 + 228 WP_002906690.1 tautomerase PptA -
GPJ73_RS12815 2577280..2577597 + 318 WP_021469727.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GPJ73_RS12820 2577673..2577969 + 297 WP_020804705.1 helix-turn-helix domain-containing protein Antitoxin
GPJ73_RS12825 2578048..2578494 + 447 WP_032435212.1 hypothetical protein -
GPJ73_RS12830 2578535..2580151 - 1617 WP_004175961.1 carbohydrate porin -
GPJ73_RS12835 2580195..2581577 - 1383 WP_004151222.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-2

(43-102)

Antitoxin

Cro/C1-type helix-turn-helix domain

(39-75)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12221.10 Da        Isoelectric Point: 10.2217

>T144595 WP_021469727.1 NZ_CP046612:2577280-2577597 [Klebsiella pneumoniae]
MLTFIELQGFSKRRQVLLQDDEFRAFQEILINDPEAGDTISGTGGFRKIRWSRPGMGKRGGVRVIYYNVTKKGRIYLALI
YPKNEQDELNQEQKKMLRQVADLIN

Download         Length: 318 bp

>T144595 NZ_CP062165:c1539374-1539039 [Paenibacillus sp. JNUCC-31]
TTGATTATTCCCAAGCGAGGCGATCTGATATGGCTTGACTTTGATCCTCAAGCGGGTCATGAGCAAGCCGGGAGACGACC
AGCTATTGTTCTATCGGAACATGAATTCAATGAAGTAACAGGTTTTGCCGTTGTATGTCCCATAACGAGCCAAGCCAAGG
ATTATCCTTTTGAAGTTACACTACCAGAAGACCTGCCATTTTCAGGTTTTGTTTTGACGGATCAGTTGAAGAGTCTTGAT
GTGCGGCAACGACGTATTCAAATTGCGGGGCATGCCGAACCAACCTCTGAATTTATGAAATCAGTTCTACGCAATGTCCG
TTCTATTTTGGCATAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11057.93 Da        Isoelectric Point: 6.7277

>AT144595 WP_020804705.1 NZ_CP046612:2577673-2577969 [Klebsiella pneumoniae]
MKDELFADLLASAEEMVRIEKGEQMLMEQHVHTFSEIDVKAIREATGLKQQDFAAAVGVSYELVKSWETKRRHPTGAARK
LLLLLQGNPLIINLLKVV

Download         Length: 297 bp

>AT144595 NZ_CP062165:c1539637-1539371 [Paenibacillus sp. JNUCC-31]
ATGACTACAGCCACACTGAGCAAATGGGGAAATAGCAGTGCAATTCGCATTCCTAACCAATTGCTTAAACGTCTTAATCT
GGAGGAAGGCTCGGAAATTGAGATTTTGGTGACAGCAGATAATGAATTGTTGTTGCGTCCAAAAACCAAGCCTGTAGAGT
CCAATGAGGAACTACGTAACCATCTAAAGACTTTGCTCTCTAAAGTGAAACAAGATACAAGACATGAAGAAATAGACTTT
GGCACGGAAGGGAACGAGTTACTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A331C6E2

Antitoxin

Source ID Structure
AlphaFold DB A0A6B0N7G3

References