Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) itaRT/DUF1778(antitoxin)
Location 2194838..2195675 Replicon chromosome
Accession NZ_CP046539
Organism Escherichia coli strain FHI_NMBU_10

Toxin (Protein)


Gene name itaT Uniprot ID Q3Z4X7
Locus tag GOE21_RS11070 Protein ID WP_000227784.1
Coordinates 2195133..2195675 (+) Length 181 a.a.

Antitoxin (Protein)


Gene name itaR Uniprot ID I2UQS9
Locus tag GOE21_RS11065 Protein ID WP_001297137.1
Coordinates 2194838..2195149 (+) Length 104 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GOE21_RS11040 2189858..2190805 + 948 WP_001239441.1 cytochrome o ubiquinol oxidase subunit II -
GOE21_RS11045 2190827..2192818 + 1992 WP_000467180.1 cytochrome o ubiquinol oxidase subunit I -
GOE21_RS11050 2192808..2193422 + 615 WP_000179819.1 cytochrome o ubiquinol oxidase subunit III -
GOE21_RS11055 2193422..2193751 + 330 WP_000019869.1 cytochrome o ubiquinol oxidase subunit IV -
GOE21_RS11060 2193763..2194653 + 891 WP_000971336.1 heme o synthase -
GOE21_RS11065 2194838..2195149 + 312 WP_001297137.1 DUF1778 domain-containing protein Antitoxin
GOE21_RS11070 2195133..2195675 + 543 WP_000227784.1 GNAT family N-acetyltransferase Toxin
GOE21_RS11075 2195731..2196666 - 936 WP_001365761.1 sel1 repeat family protein -
GOE21_RS11080 2197074..2198438 + 1365 WP_001000974.1 MFS transporter -
GOE21_RS11085 2198566..2199057 - 492 WP_001138904.1 nucleotide binding protein YajQ -
GOE21_RS11090 2199225..2200136 + 912 WP_000705874.1 2-dehydropantoate 2-reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(68-156)

Antitoxin

Vibrio phage ICP1, Orf50

(14-97)


Sequences

Toxin        


Download         Length: 181 a.a.        Molecular weight: 19805.02 Da        Isoelectric Point: 8.3395

>T144481 WP_000227784.1 NZ_CP046539:2195133-2195675 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Download         Length: 543 bp

>T144481 NZ_CP061975:1470333-1470815 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 104 a.a.        Molecular weight: 11358.04 Da        Isoelectric Point: 10.4825

>AT144481 WP_001297137.1 NZ_CP046539:2194838-2195149 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Download         Length: 312 bp

>AT144481 NZ_CP061975:1470079-1470345 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7BYY

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y6B3

References