Detailed information of TA system TA144330

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4798122..4798711
• Replicon: chromosome
• Accession: NZ_CP046479
• Organism: Xanthomonas oryzae pv. oryzae strain IXO842

Toxin (Protein)

• Gene name: graT
• Uniprot ID: G7TIR7
• Locus tag: IXO842_RS22395
• Protein ID: WP_012443754.1
• Coordinates: 4798430..4798711 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: IXO842_RS22390
• Protein ID: WP_011260746.1
• Coordinates: 4798122..4798412 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IXO842_RS22360 (IXO842_022405)	4793406..4793882	-	477	WP_011260741.1	DUF4123 domain-containing protein	-
IXO842_RS22365 (IXO842_022410)	4793885..4794307	-	423	WP_033013301.1	PAAR domain-containing protein	-
IXO842_RS22370 (IXO842_022415)	4794457..4794762	-	306	WP_241847213.1	hypothetical protein	-
IXO842_RS22375 (IXO842_022420)	4795017..4795349	+	333	WP_011260743.1	hypothetical protein	-
IXO842_RS22380	4795509..4795696	+	188	Protein_4272	hypothetical protein	-
IXO842_RS22385 (IXO842_022430)	4795799..4797193	-	1395	WP_011260745.1	type III secretion system effector XopQ	-
IXO842_RS22390 (IXO842_022435)	4798122..4798412	-	291	WP_011260746.1	HigA family addiction module antitoxin	Antitoxin
IXO842_RS22395 (IXO842_022440)	4798430..4798711	-	282	WP_012443754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
IXO842_RS22400 (IXO842_022445)	4798806..4801058	-	2253	WP_075239171.1	exodeoxyribonuclease V subunit alpha	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11066.83 Da
• Isoelectric Point: 10.5318

>T144330 WP_012443754.1 NZ_CP046479:c4798711-4798430 [Xanthomonas oryzae pv. oryzae]
VIRSFIDKDAEKIWLGERSRRLPADIQLVARRKLRMLNAAAHLDDLRIPPANRLEALKGQRRGQYSIRINDQWRICFRWM
EGDVVQVEIVDYH

Nucleotide

• Length: 282 bp

>T144330 NZ_CP061852:521833-522183 [Bacillus amyloliquefaciens]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAATGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10420.07 Da
• Isoelectric Point: 7.2040

>AT144330 WP_011260746.1 NZ_CP046479:c4798412-4798122 [Xanthomonas oryzae pv. oryzae]
MTVLPNIHPGDILLEEFLEPLGISQNALARATGVPPRRINEIVLGKRGITADTAVRLAAALGTSERFWLGLQADYELEQA
HRALGDLPARIKRLAA

Nucleotide

• Length: 291 bp

>AT144330 NZ_CP061852:521546-521827 [Bacillus amyloliquefaciens]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• AlphaFold DB: A0A0K0GFW5