Detailed information of TA system TA144071

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-couple_hipB
• Location: 1101292..1102529
• Replicon: chromosome
• Accession: NZ_CP046354
• Organism: Streptococcus pneumoniae strain 310

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: -
• Locus tag: GLO74_RS05680
• Protein ID: WP_000405365.1
• Coordinates: 1101292..1102053 (-)
• Length: 254 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: -
• Locus tag: GLO74_RS05685
• Protein ID: WP_000579599.1
• Coordinates: 1102053..1102529 (-)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GLO74_RS05660	1097134..1097499	-	366	WP_000263051.1	MobC family plasmid mobilization relaxosome protein	-
GLO74_RS05665	1097509..1097868	-	360	WP_000003745.1	hypothetical protein	-
GLO74_RS05670	1098778..1100034	+	1257	WP_197097166.1	ISL3 family transposase	-
GLO74_RS05675	1100108..1101264	-	1157	Protein_1072	hypothetical protein	-
GLO74_RS05680	1101292..1102053	-	762	WP_000405365.1	zeta toxin family protein	Toxin
GLO74_RS05685	1102053..1102529	-	477	WP_000579599.1	helix-turn-helix domain-containing protein	Antitoxin
GLO74_RS05690	1102974..1103429	-	456	WP_000500465.1	hypothetical protein	-
GLO74_RS05695	1103704..1105443	-	1740	WP_000423466.1	glycoside hydrolase family 13 protein	-
GLO74_RS05700	1105606..1106490	-	885	WP_000710115.1	diacylglycerol kinase family lipid kinase	-
GLO74_RS05705	1106602..1107456	-	855	WP_024477726.1	ADP-ribosylglycohydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	erm(B) / tet(M)	-	1076300..1205679	129379
	-	flank	IS/Tn	-	-	1098778..1100034	1256

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (25-215)

Antitoxin

• Cro/C1-type helix-turn-helix domain (3-60)

Sequences

Toxin

Protein

• Length: 254 a.a.
• Molecular weight: 29060.05 Da
• Isoelectric Point: 6.8511

>T144071 WP_000405365.1 NZ_CP046354:c1102053-1101292 [Streptococcus pneumoniae]
MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAGKTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQ
EYGKDSVEYTKDFAGKMVESLVTKLSSLRYNLLIEGTLRTVDVPKKTAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFESIQIYQRDRSCVYDSKENTTSAADVLQELLFGEWSQVEKEML
QVGEKRLNELLEK

Nucleotide

• Length: 762 bp

>T144071 NZ_CP061702:62603-63085 [Klebsiella pneumoniae]
ATGGGAATGAGGCCTCCGGAACCTCTTACCCCTGAACACAACATTGCTGATTTTTGTTGTCAGGATCAGGTTCTTTCTGA
GTGGTTGAAGAAAAAGGCGCTGAAGAACCATAGCACGGGCCTTTCAAGAGTTTTCGTCGTCTGTGCTGAAAATACGAATA
GGGTAATTGCGTACTACTGTTTGGCATCCGGAAGCGTGCACAGAAACACCGTACCCGGAGCATACCGTCGTAATGCACCA
GAGGCTTTACCGGTCATTGTTCTGGGAAGGCTTGCGGTTGACGCTGCCTGGGCAAGAAAAGGGCTGGGAGCTGCACTGCT
TAAGGATGCGATATACCGAACACAACATATTGCAATACAGGTTGGTGTTAGGGCGCTTCTTGTTCACGCCTTAAATGATG
AAGTTAGGGAATTTTACACAAAGTTTGGATTCGAGCCCTCCATTGCCAATGCGCTGACATTATTATTTCCGATCAAAACT
TGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 18251.60 Da
• Isoelectric Point: 4.3571

>AT144071 WP_000579599.1 NZ_CP046354:c1102529-1102053 [Streptococcus pneumoniae]
MIGKNIKSLRKTHDLTQDDFSRIVGISRNSLSRYENGTSSVSTELIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIE
IVKERGANLLSRLYRYQDSQGISIDDESNPWILMSDDLSDLIHTNIYLVETFDEIERYSGYLDGIERMLEISEKRMVA

Nucleotide

• Length: 477 bp

>AT144071 NZ_CP061702:62334-62612 [Klebsiella pneumoniae]
ATGAAACCAGACATTCGTGAGGCTCCGATAAACATCCGGGCGAAAACATCTCAACGCGATCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTGGAAGTGGCCTGCCGCGAAGCGCAGGACATCTTGTTAGATCAGCGCT
TGTTTCTGCTGGATGATAAGCAGTTTAACGCGTTTGTCGAAGAGCTTGACGCCCCTATGACCCCAGAGCGTCAGGCGCGT
ATTGATAACCTGATGAACCGCAAATCACCATGGGAATGA