Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3342703..3343501 Replicon chromosome
Accession NZ_CP046259
Organism Escherichia coli strain ECO2947

Toxin (Protein)


Gene name yeeV Uniprot ID A0A0V7WHV8
Locus tag GK046_RS16140 Protein ID WP_001506713.1
Coordinates 3343124..3343501 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A093DMQ4
Locus tag GK046_RS16135 Protein ID WP_001280952.1
Coordinates 3342703..3343077 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GK046_RS16095 3337865..3338646 + 782 Protein_3129 IS3 family transposase -
GK046_RS16105 3339954..3340391 + 438 Protein_3131 DDE-type integrase/transposase/recombinase -
GK046_RS16110 3340401..3340952 + 552 Protein_3132 DUF945 domain-containing protein -
GK046_RS16115 3340952..3341197 + 246 WP_001164975.1 hypothetical protein -
GK046_RS16120 3341291..3341764 + 474 WP_001368734.1 antirestriction protein -
GK046_RS16125 3341780..3342256 + 477 WP_069723416.1 RadC family protein -
GK046_RS16130 3342319..3342540 + 222 WP_000692311.1 DUF987 domain-containing protein -
GK046_RS16135 3342703..3343077 + 375 WP_001280952.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GK046_RS16140 3343124..3343501 + 378 WP_001506713.1 TA system toxin CbtA family protein Toxin
GK046_RS16145 3343498..3343986 + 489 WP_001470575.1 hypothetical protein -
GK046_RS16150 3343998..3344195 + 198 WP_000839249.1 DUF957 domain-containing protein -
GK046_RS24780 3344274..3344432 + 159 WP_001467148.1 hypothetical protein -
GK046_RS16160 3344896..3345606 + 711 WP_001219054.1 integrase arm-type DNA-binding domain-containing protein -
GK046_RS16165 3345610..3346272 + 663 WP_000357928.1 hypothetical protein -
GK046_RS24785 3346325..3346492 - 168 WP_001356018.1 hypothetical protein -
GK046_RS16170 3346786..3347703 - 918 WP_125090762.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
GK046_RS16175 3347696..3348469 - 774 WP_000157137.1 type IV toxin-antitoxin system AbiEi family antitoxin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - espK / espF / escG / escF / cesD2 / espB / espD / espA / sepL / escD / eae / cesT / tir / map / cesF / espH / sepQ/escQ / escO / escN / escV / cesL / escI / escJ / sepD / escC / cesD / etgA / escU / escT / escS / escR / escL / escK / cesAB / escE / espG 3285795..3345606 59811


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-118)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14178.26 Da        Isoelectric Point: 7.8045

>T143772 WP_001506713.1 NZ_CP046259:3343124-3343501 [Escherichia coli]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYITVNNITLGKHPEAKR

Download         Length: 378 bp

>T143772 NZ_CP061350:c5170599-5170216 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACTTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13719.50 Da        Isoelectric Point: 6.6249

>AT143772 WP_001280952.1 NZ_CP046259:3342703-3343077 [Escherichia coli]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRNQHTVTLYAKGLTCEADTLGSCGYVYLAVYPAQAPATTS

Download         Length: 375 bp

>AT143772 NZ_CP061350:c5170841-5170599 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V7WHV8

Antitoxin

Source ID Structure
AlphaFold DB A0A093DMQ4

References