Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 2623788..2624623 Replicon chromosome
Accession NZ_CP046259
Organism Escherichia coli strain ECO2947

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2Y0VZS4
Locus tag GK046_RS12670 Protein ID WP_032304079.1
Coordinates 2624246..2624623 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A369FMF5
Locus tag GK046_RS12665 Protein ID WP_024188924.1
Coordinates 2623788..2624156 (+) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GK046_RS24755 2618912..2619079 - 168 WP_001300931.1 hypothetical protein -
GK046_RS12640 2620482..2621158 - 677 Protein_2472 transposase -
GK046_RS12645 2621146..2621619 + 474 WP_155430294.1 antirestriction protein -
GK046_RS12650 2621635..2622111 + 477 WP_001186725.1 RadC family protein -
GK046_RS12655 2622264..2623477 + 1214 WP_173662722.1 IS3 family transposase -
GK046_RS12660 2623520..2623714 + 195 WP_155430295.1 DUF987 domain-containing protein -
GK046_RS12665 2623788..2624156 + 369 WP_024188924.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
GK046_RS12670 2624246..2624623 + 378 WP_032304079.1 TA system toxin CbtA family protein Toxin
GK046_RS12675 2624620..2625108 + 489 WP_000761714.1 hypothetical protein -
GK046_RS12680 2625120..2625317 + 198 WP_000839243.1 DUF957 domain-containing protein -
GK046_RS12685 2625402..2626247 + 846 WP_000065752.1 DUF4942 domain-containing protein -
GK046_RS12695 2627039..2628223 + 1185 WP_001172876.1 sugar efflux transporter -
GK046_RS12700 2628334..2629257 + 924 WP_000535960.1 carboxylate/amino acid/amine transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(19-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14102.08 Da        Isoelectric Point: 7.9094

>T143767 WP_032304079.1 NZ_CP046259:2624246-2624623 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQLGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Download         Length: 378 bp

>T143767 NZ_CP061350:c1578951-1578733 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13633.45 Da        Isoelectric Point: 6.7386

>AT143767 WP_024188924.1 NZ_CP046259:2623788-2624156 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQKGNRLHYLADRAGIRGRFSDADTYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKT

Download         Length: 369 bp

>AT143767 NZ_CP061350:c1579352-1578978 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Y0VZS4

Antitoxin

Source ID Structure
AlphaFold DB A0A369FMF5

References