Detailed information of TA system TA143680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 1058529..1059666
• Replicon: chromosome
• Accession: NZ_CP046123
• Organism: Enterococcus casseliflavus strain EC291

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: GFU50_RS04990
• Protein ID: WP_084679662.1
• Coordinates: 1058803..1059666 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: -
• Locus tag: GFU50_RS04985
• Protein ID: WP_126347911.1
• Coordinates: 1058529..1058801 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFU50_RS04970	1055550..1057226	+	1677	WP_082207593.1	type IA DNA topoisomerase	-
GFU50_RS04975	1057308..1058204	+	897	WP_002333001.1	ParA family protein	-
GFU50_RS04980	1058302..1058511	+	210	WP_000527318.1	peptide-binding protein	-
GFU50_RS04985	1058529..1058801	+	273	WP_126347911.1	antitoxin	Antitoxin
GFU50_RS04990	1058803..1059666	+	864	WP_084679662.1	zeta toxin family protein	Toxin
GFU50_RS04995	1060106..1060423	+	318	WP_002326830.1	hypothetical protein	-
GFU50_RS05000	1060956..1061453	+	498	WP_002300565.1	molecular chaperone DnaJ	-
GFU50_RS05005	1061487..1061792	+	306	WP_000351790.1	hypothetical protein	-
GFU50_RS05010	1061815..1062072	-	258	WP_033918709.1	hypothetical protein	-
GFU50_RS05015	1062075..1062374	-	300	WP_002332653.1	hypothetical protein	-
GFU50_RS05020	1062666..1064651	+	1986	WP_154694273.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	895661..1083734	188073

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-214)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32437.91 Da
• Isoelectric Point: 6.1573

>T143680 WP_084679662.1 NZ_CP046123:1058803-1059666 [Enterococcus casseliflavus]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVVVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKIYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPNNLETLQKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T143680 NZ_CP046123:1058803-1059666 [Enterococcus casseliflavus]
ATGGCAAATATAGTCAATTTTACTGACAAACAGTTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGAAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTGTTGTCATTGATAATGATACCTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTTTATGAAAAAGACGTGGTAAAACACGTTACCCCTTATTCTAATCGTATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGAACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATATACGTCATGGCAGTACCGAAAATTGAATCATACTTAGGAACGATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCACATGATATTGTGGTTAAAAATTTACCTAA
CAATTTAGAAACCCTTCAAAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAGACACCTAAACTTCCAGGACTTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10735.36 Da
• Isoelectric Point: 8.3744

>AT143680 WP_126347911.1 NZ_CP046123:1058529-1058801 [Enterococcus casseliflavus]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLKQLKLAKRVNLFDYSLEELKAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT143680 NZ_CP046123:1058529-1058801 [Enterococcus casseliflavus]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAATCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAAACAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTAAAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA