Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 1058529..1059666 Replicon chromosome
Accession NZ_CP046123
Organism Enterococcus casseliflavus strain EC291

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag GFU50_RS04990 Protein ID WP_084679662.1
Coordinates 1058803..1059666 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID -
Locus tag GFU50_RS04985 Protein ID WP_126347911.1
Coordinates 1058529..1058801 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFU50_RS04970 1055550..1057226 + 1677 WP_082207593.1 type IA DNA topoisomerase -
GFU50_RS04975 1057308..1058204 + 897 WP_002333001.1 ParA family protein -
GFU50_RS04980 1058302..1058511 + 210 WP_000527318.1 peptide-binding protein -
GFU50_RS04985 1058529..1058801 + 273 WP_126347911.1 antitoxin Antitoxin
GFU50_RS04990 1058803..1059666 + 864 WP_084679662.1 zeta toxin family protein Toxin
GFU50_RS04995 1060106..1060423 + 318 WP_002326830.1 hypothetical protein -
GFU50_RS05000 1060956..1061453 + 498 WP_002300565.1 molecular chaperone DnaJ -
GFU50_RS05005 1061487..1061792 + 306 WP_000351790.1 hypothetical protein -
GFU50_RS05010 1061815..1062072 - 258 WP_033918709.1 hypothetical protein -
GFU50_RS05015 1062075..1062374 - 300 WP_002332653.1 hypothetical protein -
GFU50_RS05020 1062666..1064651 + 1986 WP_154694273.1 MobA/MobL family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 895661..1083734 188073


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32437.91 Da        Isoelectric Point: 6.1573

>T143680 WP_084679662.1 NZ_CP046123:1058803-1059666 [Enterococcus casseliflavus]
MANIVNFTDKQFENRLNDNLEELVQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVVVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEALISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKIYVMAVPKIESYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPNNLETLQKTGLFSDIRLYNREGVKLYSSLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMVQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

>T143680 NZ_CP046123:1058803-1059666 [Enterococcus casseliflavus]
ATGGCAAATATAGTCAATTTTACTGACAAACAGTTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGGTTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGAAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTGTTGTCATTGATAATGATACCTTCAAACAACAGCACCCAAATTTTGACGAATTAGTG
AAACTTTATGAAAAAGACGTGGTAAAACACGTTACCCCTTATTCTAATCGTATGACAGAAGCACTTATTAGCCGTTTAAG
CGATCAAGGCTATAATTTAGTGATTGAAGGAACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATATACGTCATGGCAGTACCGAAAATTGAATCATACTTAGGAACGATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCACATGATATTGTGGTTAAAAATTTACCTAA
CAATTTAGAAACCCTTCAAAAAACGGGCTTATTTAGCGATATAAGGCTTTACAATAGAGAAGGAGTGAAACTCTATTCAA
GCTTAGAAACGCCTTCCATTAGTCCGAAAGAGACCTTAGAAAGAGAATTAAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTGGAGAGAATAGAGCAAAAAATGGTTCAAAATCAACACCAAGAAACCCCTGAATTTAAAGCAATTCAACAAAA
AATGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAGACACCTAAACTTCCAGGACTTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10735.36 Da        Isoelectric Point: 8.3744

>AT143680 WP_126347911.1 NZ_CP046123:1058529-1058801 [Enterococcus casseliflavus]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLKQLKLAKRVNLFDYSLEELKAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT143680 NZ_CP046123:1058529-1058801 [Enterococcus casseliflavus]
ATGGCAGTTACGTATGAAAAAACTTTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAATCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAAACAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTAAAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References