Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/DUF433(antitoxin)
Location 766478..767037 Replicon chromosome
Accession NZ_CP046058
Organism Microcystis aeruginosa NIES-298

Toxin (Protein)


Gene name vapC Uniprot ID I4GP34
Locus tag D3800_RS03545 Protein ID WP_002755563.1
Coordinates 766699..767037 (+) Length 113 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1X9LGK3
Locus tag D3800_RS03540 Protein ID WP_002740734.1
Coordinates 766478..766702 (+) Length 75 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D3800_RS03520 762551..763828 + 1278 WP_002755549.1 adenosylhomocysteinase -
D3800_RS03525 763963..764568 + 606 WP_002769621.1 DedA family protein -
D3800_RS03530 764713..765291 + 579 WP_002773172.1 hypothetical protein -
D3800_RS03535 765614..765838 - 225 WP_103112140.1 hypothetical protein -
D3800_RS03540 766478..766702 + 225 WP_002740734.1 DUF433 domain-containing protein Antitoxin
D3800_RS03545 766699..767037 + 339 WP_002755563.1 DUF5615 family PIN-like protein Toxin
D3800_RS03550 767277..768182 - 906 WP_002755565.1 metal ABC transporter permease -
D3800_RS03555 768318..768836 + 519 WP_002734244.1 cofactor assembly of complex C subunit B -
D3800_RS03560 768844..769260 + 417 WP_103112141.1 acyl-CoA thioesterase -
D3800_RS03565 769264..769893 + 630 WP_103112142.1 DUF1361 domain-containing protein -
D3800_RS03570 770020..771360 + 1341 WP_103112143.1 Rieske 2Fe-2S domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Domain of unknown function DUF5615

(1-78)

Antitoxin

Protein of unknown function DUF433

(8-63)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 12303.09 Da        Isoelectric Point: 4.1314

>T143474 WP_002755563.1 NZ_CP046058:766699-767037 [Microcystis aeruginosa NIES-298]
MTIWVDAHLSPAIATWISTTLEIEAVALRDLGLRDAEDTEIFQVAKAQRAILMTKDSDFVDLVERLGSPPQIIWLTCGNT
SNARLREILSETLPRALELLAAGETLVEISGD

Download         Length: 339 bp

>T143474 NZ_CP061108:1115897-1116208 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACACCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAGATATC
GCAACAAATGGTACGTTCTGGATGTCGGCGGGAACAATCTTAGGGTTATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACGAATCACGCAGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin


Download         Length: 75 a.a.        Molecular weight: 8134.55 Da        Isoelectric Point: 6.9476

>AT143474 WP_002740734.1 NZ_CP046058:766478-766702 [Microcystis aeruginosa NIES-298]
MAELLSRITVNPRQCGGRACIRGMRIRVSDVLDLFAAGLSAREILDEMPDLEADDLKAALLYASRKLNHPVLLA

Download         Length: 225 bp

>AT143474 NZ_CP061108:1116205-1116624 [Escherichia coli]
ATGACTGCTAATGCTGCACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGGCGAGCCGCATTGCAGAGTATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCGGTT
GGCGTGGCGTTACTTCGCACGTTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGTCAGATTTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A822LFQ3

Antitoxin

Source ID Structure
AlphaFold DB A0A1X9LGK3

References