Detailed information of TA system TA143360

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3466109..3466946
• Replicon: chromosome
• Accession: NZ_CP046009
• Organism: Escherichia coli strain 1919D62

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: -
• Locus tag: GJD96_RS18310
• Protein ID: WP_112978798.1
• Coordinates: 3466404..3466946 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: GJD96_RS18305
• Protein ID: WP_001297137.1
• Coordinates: 3466109..3466420 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD96_RS18280	3461129..3462076	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
GJD96_RS18285	3462098..3464089	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
GJD96_RS18290	3464079..3464693	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
GJD96_RS18295	3464693..3465022	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
GJD96_RS18300	3465034..3465924	+	891	WP_000971336.1	heme o synthase	-
GJD96_RS18305	3466109..3466420	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
GJD96_RS18310	3466404..3466946	+	543	WP_112978798.1	GNAT family N-acetyltransferase	Toxin
GJD96_RS18315	3467002..3467937	-	936	WP_096310067.1	sel1 repeat family protein	-
GJD96_RS18320	3468345..3469709	+	1365	WP_001000978.1	MFS transporter	-
GJD96_RS18325	3469837..3470328	-	492	WP_001138904.1	nucleotide binding protein YajQ	-
GJD96_RS18330	3470496..3471407	+	912	WP_000705853.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19776.97 Da
• Isoelectric Point: 8.3395

>T143360 WP_112978798.1 NZ_CP046009:3466404-3466946 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGAYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T143360 NZ_CP061062:c3931128-3930595 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT143360 WP_001297137.1 NZ_CP046009:3466109-3466420 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT143360 NZ_CP061062:c3931405-3931139 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0E0Y6B3