Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3655130..3655748 Replicon chromosome
Accession NZ_CP045941
Organism Shigella flexneri 2a strain AUSMDU00010535

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GJE14_RS18545 Protein ID WP_001291435.1
Coordinates 3655530..3655748 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID Q0T7C3
Locus tag GJE14_RS18540 Protein ID WP_000344797.1
Coordinates 3655130..3655504 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE14_RS18530 (3650219) 3650219..3651412 + 1194 WP_011069267.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GJE14_RS18535 (3651435) 3651435..3654584 + 3150 WP_001132484.1 efflux RND transporter permease AcrB -
GJE14_RS18540 (3655130) 3655130..3655504 + 375 WP_000344797.1 Hha toxicity modulator TomB Antitoxin
GJE14_RS18545 (3655530) 3655530..3655748 + 219 WP_001291435.1 HHA domain-containing protein Toxin
GJE14_RS18550 (3655920) 3655920..3656471 + 552 Protein_3631 maltose O-acetyltransferase -
GJE14_RS18555 (3656587) 3656587..3657057 + 471 WP_000136192.1 YlaC family protein -
GJE14_RS18560 (3657221) 3657221..3658771 + 1551 WP_005098218.1 cyclic-guanylate-specific phosphodiesterase PdeB -
GJE14_RS18565 (3658813) 3658813..3659166 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GJE14_RS18575 (3659545) 3659545..3659856 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 3659886..3661214 1328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T143081 WP_001291435.1 NZ_CP045941:3655530-3655748 [Shigella flexneri 2a]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T143081 NZ_CP060938:2488224-2488475 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14513.38 Da        Isoelectric Point: 4.9091

>AT143081 WP_000344797.1 NZ_CP045941:3655130-3655504 [Shigella flexneri 2a]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQALQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT143081 NZ_CP060938:2488472-2488726 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A4P7TPD8

References