Detailed information of TA system TA142560

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1176219..1176809
• Replicon: chromosome
• Accession: NZ_CP045783
• Organism: Klebsiella variicola strain LEMB11

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A1F2LYA2
• Locus tag: GFC07_RS05725
• Protein ID: WP_008804165.1
• Coordinates: 1176219..1176551 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: GFC07_RS05730
• Protein ID: WP_100781507.1
• Coordinates: 1176552..1176809 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GFC07_RS05700	1172257..1172502	+	246	WP_008804171.1	signal transduction protein PmrD	-
GFC07_RS05705	1172633..1174087	+	1455	WP_008804170.1	AMP nucleosidase	-
GFC07_RS05715	1174460..1175896	-	1437	WP_042948427.1	EmmdR/YeeO family multidrug/toxin efflux MATE transporter	-
GFC07_RS05725	1176219..1176551	-	333	WP_008804165.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GFC07_RS05730	1176552..1176809	-	258	WP_100781507.1	antitoxin	Antitoxin
GFC07_RS05735	1177338..1178438	+	1101	WP_100781500.1	phosphotransferase	-
GFC07_RS05740	1178435..1179406	-	972	WP_087499192.1	sensor domain-containing diguanylate cyclase	-
GFC07_RS05745	1179613..1180449	-	837	WP_008804155.1	alpha/beta hydrolase	-
GFC07_RS05750	1180628..1181203	-	576	WP_012541129.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11867.71 Da
• Isoelectric Point: 10.1839

>T142560 WP_008804165.1 NZ_CP045783:c1176551-1176219 [Klebsiella variicola]
MDRGEIWLVSLDPIAGHEQSGKRPVLIVSKASFNKLTRLPVVVPVTSGGNFARTAGFTVSLEEAGTKTTGVIRCDQPRTI
DMAARNGKRLERIPDAVVNEVLARLDAILS

Nucleotide

• Length: 333 bp

>T142560 NZ_CP060733:c1089379-1089161 [Enterobacter roggenkampii]
ATGTCTGATAAACCACTCACTAAAATCGATTATTTGATGCGCTTACGACGTTGTCAGTCAATTGACACCCTCGAACGCGT
GATTGAAAAGAATAAATACGAGCTTTCTGATAATGAACTGGCCGTATTTTATTCAGCCGCTGACCATCGTCTTGCTGAGC
TGACCATGAATAAGTTGTATGACAAAATCCCTACTTCTGTATGGAAATTTGTCCGTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9392.86 Da
• Isoelectric Point: 4.3556

>AT142560 WP_100781507.1 NZ_CP045783:c1176809-1176552 [Klebsiella variicola]
MYKTRLKKVGGSIMLAVPPAVLKALELSTDSEVGMTIDNGCLIIEPQKRPHYSLEELLAQCDPHAEMSEEDQEWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT142560 NZ_CP060733:c1089781-1089407 [Enterobacter roggenkampii]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAACCTTGATGAAAGCAACCATGGCTGGGTAAACGACCCAACGTCTGCCATCAATTTACAGTTGAATGAGCTGATAGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGCTCATTGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGTAGTTACGGCATTAACGCCCAGGATTTGCAAAAATGGCGTAAATCGGGAAACCGGCT
ATTCCGCTGTTTCGTCAACGTGAGCAGAGCTAACCCGGTTAGCCTTTCCTGTTAA

Structures

Toxin

• AlphaFold DB: A0A1F2LYA2