Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4478385..4479004 Replicon chromosome
Accession NZ_CP045782
Organism Klebsiella pneumoniae strain LEMB18

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag GFB99_RS21535 Protein ID WP_002892050.1
Coordinates 4478385..4478603 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag GFB99_RS21540 Protein ID WP_002892066.1
Coordinates 4478630..4479004 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFB99_RS21505 4474432..4474695 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
GFB99_RS21510 4474695..4474835 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
GFB99_RS21515 4474832..4475530 - 699 WP_102001050.1 GNAT family N-acetyltransferase -
GFB99_RS21520 4475631..4477082 + 1452 WP_102001051.1 PLP-dependent aminotransferase family protein -
GFB99_RS21525 4477057..4477527 - 471 WP_002892026.1 YlaC family protein -
GFB99_RS25435 4477548..4477688 + 141 WP_004147370.1 hypothetical protein -
GFB99_RS21530 4477660..4478226 - 567 WP_002892030.1 maltose O-acetyltransferase -
GFB99_RS21535 4478385..4478603 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
GFB99_RS21540 4478630..4479004 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
GFB99_RS21545 4479490..4482636 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
GFB99_RS21550 4482659..4483852 - 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T142549 WP_002892050.1 NZ_CP045782:c4478603-4478385 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T142549 NZ_CP060726:c4501444-4501160 [Providencia rettgeri]
ATGAATACTAAATTAAAAGAATTTGATATCGCTGAGTATTTAAACAGTGAAGAAGAAATACAAATGTATCTAAACGAAAT
TCTAGAAGAAGGTGACCCTAGTCTAGTTCTCGCTGCACTTGGTGATATTGCGAAGGCAAGAAATATGAGCCAATTAGCTA
AAGAAGTTGGAATGAGTCGTGAAGGTTTATATAAGGCGCTATCAGGACAGGGAAATCCAACATTTATGACGATTATAAAA
ATCGTTAATTCTCTCGGATTAAAGCTACAGTTTAAGAGTATCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT142549 WP_002892066.1 NZ_CP045782:c4479004-4478630 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT142549 NZ_CP060726:c4501743-4501441 [Providencia rettgeri]
ATGATCACGGTTCTAACAACGGAATGTTTTGATAGTTGGATCAAAAATCTTAGAGATATTCGAGCTAAAACTAAAATTCT
AATGAGAATTAGGCGGTTAAAAAATGGAAATTATGGTGATGTTCAACCCATTGGTGATGGCTTTTCGGAACTGAGGGTAC
ATGAAGGACAAGGGTATCGTGTTTATCTAAAGCAGAAAAACAATGTTATTGTAATTTTACTTTGTGGTGGGACAAAGGCA
ACGCAGCAGAAGGATATCCATAAAGCTAAACTCCTTTTTGGTGAAGTTGAGGGGGAACTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References