Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3273115..3273740 Replicon chromosome
Accession NZ_CP045782
Organism Klebsiella pneumoniae strain LEMB18

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag GFB99_RS15880 Protein ID WP_002882817.1
Coordinates 3273357..3273740 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag GFB99_RS15875 Protein ID WP_004150355.1
Coordinates 3273115..3273357 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFB99_RS15850 3269106..3269705 + 600 WP_004151865.1 glucose-1-phosphatase -
GFB99_RS15855 3269699..3270559 + 861 WP_004146232.1 virulence factor BrkB family protein -
GFB99_RS15860 3270556..3270993 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
GFB99_RS15865 3271038..3271979 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
GFB99_RS15870 3271993..3272910 - 918 WP_004187175.1 alpha/beta hydrolase -
GFB99_RS15875 3273115..3273357 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
GFB99_RS15880 3273357..3273740 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
GFB99_RS15885 3273914..3274843 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
GFB99_RS15890 3274840..3275475 - 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
GFB99_RS15895 3275472..3276374 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T142545 WP_002882817.1 NZ_CP045782:3273357-3273740 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T142545 NZ_CP060726:2502444-2502902 [Providencia rettgeri]
ATGATTTTATATCGATTGGTTAAATCCAATTTTGCGCATGATGCATGGTCGGGCCAAGGAGCTATGCTCTACGGCGGGCG
TTGGAATCATAAAGGGACTCCTGCTGTTTATACTTCAACGAGTATTTCATTAGCGACCCTTGAAGTACTCGTTCACATCA
ATCAAGACATACTATTGAGCCAGTTCAGCTTACTATCCATAGATATCAATGATAAGTACGTGATGAAATTGTCGTTAGAT
CACCTTCCGACAGATTGGCAACAAGACCCCGCACCAACCTCCACCATGGATATTGGCACTATATGGCTTAATAACCAAGA
TAGTCTAGCATTATTAATTCCCTCTTGTATTGTTCCCTATGAATACAACGCAATTATTAACCCGTTACATCCGCAATTTC
AAAAAGCTTTACATACCGTTAAACCACTAGATTTTAGTGTTGACCCGCGCTTAGCATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT142545 WP_004150355.1 NZ_CP045782:3273115-3273357 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT142545 NZ_CP060726:2501986-2502447 [Providencia rettgeri]
ATGAGAAACTATATCCCCACAACCCCACTTCAACCGACTGCTTCATCCTTTAAACCACTGTGGCGCCATGTAGGGTTACC
CGCAGAACGTGGTATTGAGTTAACTCATTTCTTGCGTCAAGGCCTATCTGTCTCTGTCTTAGACAATATCCAAGAATGGT
CAGGAATGAGCAAAACTGAATTATTGCGAATATCCGGCATTAATGAGCGCAATATTGCTCGACGTAAAAATGCTGGCCAG
CCCCTTAATGCTGATGAAAGTGAGAGAATTGCTCGTCTAGTGCGGGTATTCGATGCTGCGGTGCGTTTATTTAATGGAGA
TAAGCACGCGGCAAGTGATTGGCTTAACCACCCAGTTAAAGGGTTAGGACATTTACGCCCTATTGAGCTTATTGCGACAG
AAAGCGGAGCCATTGAAGTGATTGATTTGATTGGCCGTATTGAACATGGCATATTCTCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References