Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-VapI
Location 4387195..4387790 Replicon chromosome
Accession NZ_CP045768
Organism Pseudomonas aeruginosa strain CFSAN084950

Toxin (Protein)


Gene name higB Uniprot ID -
Locus tag GFD20_RS20140 Protein ID WP_128570772.1
Coordinates 4387512..4387790 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GFD20_RS20135 Protein ID WP_003113527.1
Coordinates 4387195..4387500 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GFD20_RS20115 4383744..4384211 - 468 WP_124075005.1 hypothetical protein -
GFD20_RS20120 4384208..4384726 - 519 WP_153655584.1 hypothetical protein -
GFD20_RS20130 4386020..4386547 - 528 WP_153655585.1 hypothetical protein -
GFD20_RS20135 4387195..4387500 - 306 WP_003113527.1 HigA family addiction module antidote protein Antitoxin
GFD20_RS20140 4387512..4387790 - 279 WP_128570772.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GFD20_RS20145 4388119..4390347 + 2229 WP_128570773.1 TonB-dependent siderophore receptor -
GFD20_RS20150 4390417..4391064 - 648 WP_003095021.1 carbonate dehydratase -
GFD20_RS20155 4391126..4392364 - 1239 WP_153655586.1 C69 family dipeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(5-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(22-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10631.22 Da        Isoelectric Point: 7.8937

>T142499 WP_128570772.1 NZ_CP045768:c4387790-4387512 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDLWRVCFVWTD
AGPEEVEIVDYH

Download         Length: 279 bp

>T142499 NZ_CP060709:c246238-245960 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATATTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATACTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11247.81 Da        Isoelectric Point: 5.0747

>AT142499 WP_003113527.1 NZ_CP045768:c4387500-4387195 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMEFDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Download         Length: 306 bp

>AT142499 NZ_CP060709:c246501-246241 [Escherichia coli]
ATGGCTGCTAACGCGTTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCAGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCATTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References