Detailed information of TA system TA141947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-RHH
• Location: 4400082..4400698
• Replicon: chromosome
• Accession: NZ_CP045485
• Organism: Enterobacter cloacae strain 88701

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GEA56_RS20850
• Protein ID: WP_203463916.1
• Coordinates: 4400082..4400453 (-)
• Length: 124 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A4R0FQN1
• Locus tag: GEA56_RS20855
• Protein ID: WP_063449683.1
• Coordinates: 4400456..4400698 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GEA56_RS20835	4397582..4398484	+	903	WP_003861960.1	formate dehydrogenase subunit beta	-
GEA56_RS20840	4398481..4399116	+	636	WP_015569914.1	formate dehydrogenase cytochrome b556 subunit	-
GEA56_RS20845	4399113..4400042	+	930	WP_023315053.1	formate dehydrogenase accessory protein FdhE	-
GEA56_RS20850	4400082..4400453	-	372	WP_203463916.1	type II toxin-antitoxin system VapC family toxin	Toxin
GEA56_RS20855	4400456..4400698	-	243	WP_063449683.1	ribbon-helix-helix domain-containing protein	Antitoxin
GEA56_RS20860	4400897..4401817	+	921	WP_203463917.1	alpha/beta hydrolase	-
GEA56_RS20865	4401826..4402767	-	942	WP_063449681.1	fatty acid biosynthesis protein FabY	-
GEA56_RS20870	4402812..4403249	-	438	WP_203463918.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GEA56_RS20875	4403246..4404127	-	882	WP_063449679.1	virulence factor BrkB family protein	-
GEA56_RS20880	4404121..4404720	-	600	WP_063449678.1	glucose-1-phosphatase	-
GEA56_RS20885	4404839..4405639	-	801	WP_063865422.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-114)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-49)

Sequences

Toxin

Protein

• Length: 124 a.a.
• Molecular weight: 13586.67 Da
• Isoelectric Point: 6.9790

>T141947 WP_203463916.1 NZ_CP045485:c4400453-4400082 [Enterobacter cloacae]
MEHMAVFDTNILIDLFNNRVEAADTIDRTASHRAISLITWMEVMVGARRHGHEAKTAAVMGAFEIIDVSRDIAERSVVLR
EKHGMKLPDAIILATAQSRNCPLVSRNTKDFAGIAGVVSPYQL

Nucleotide

• Length: 372 bp

>T141947 NZ_CP060243:c5677168-5676986 [Pseudomonas aeruginosa]
ATGCGGAGTAGGGAAGTGATTGATTTGCTGTTGGAGGATGGCTGGTATGAAGTGGCGGTGAAAGGTAGCCACCACCAGTT
CAAACATCCTTCAAAGCCTGGAAAGGTGACGGTTCAACATCCGTCCTCGACGATACCGAAAGGAACCCTGAACAACATAC
TGAAACAAGCCGGCCTCAAGTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9475.63 Da
• Isoelectric Point: 4.1849

>AT141947 WP_063449683.1 NZ_CP045485:c4400698-4400456 [Enterobacter cloacae]
MSLLADFEMGRILIDLSDDVIQRLDNLKQLRNQPRAELLREAIEQYLDQQSSSVIRDALGLWGNQQEDGLEYERKLREEW

Nucleotide

• Length: 243 bp

>AT141947 NZ_CP060243:c5676958-5676554 [Pseudomonas aeruginosa]
ATGAAATTCCCCGTGGTGTTACACAAAGACCCTGATTCTGACTATGGCGTGACTGTGCCCGATGTGCCGGGTTGCTTCTC
TGCCGGCGCTACGGTGTCCGAGGCCCTGGCCAATGTAGAAGAGGCGCTGGCCTTGCACTTCGAAGGCTTGGTTACGGACG
GCGAAGAGCTACCCCAGCCGCAGGACGTTGACGCCCATATGAAGAATCCGGACTTCGAAGGTGGCGTATGGGCGGTGGTT
GACTTCGACGTGACCCCGTACCTGGGCAAGGCCGTACGCTTTAATGCGACGCTCCCGGAACACCTTCTGCAGCGGATCGA
TGAGCGCGTGAAGGTGGATAAGCGCTATCAGTCTCGGTCGGGCTTTCTGGCAACAGCGGCGATGCGCGAACTGTCAGTGG
CGTGA

Structures

Antitoxin

• AlphaFold DB: A0A4R0FQN1