Detailed information of TA system TA141745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 66654..67346
• Replicon: plasmid pTHAF64_c
• Accession: NZ_CP045358
• Organism: Vibrio sp. THAF64

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: FIU99_RS27155
• Protein ID: WP_152473071.1
• Coordinates: 67062..67346 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: FIU99_RS27150
• Protein ID: WP_152473070.1
• Coordinates: 66654..66989 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU99_RS27125	62743..63171	+	429	WP_152473065.1	hypothetical protein	-
FIU99_RS27130	63158..64738	+	1581	WP_152473066.1	hypothetical protein	-
FIU99_RS27135	64759..65439	+	681	WP_152473067.1	hypothetical protein	-
FIU99_RS27140	65449..66069	+	621	WP_152473068.1	hypothetical protein	-
FIU99_RS27145	66086..66586	+	501	WP_152473069.1	baseplate protein	-
FIU99_RS27150	66654..66989	-	336	WP_152473070.1	HigA family addiction module antidote protein	Antitoxin
FIU99_RS27155	67062..67346	-	285	WP_152473071.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
FIU99_RS27160	67859..68101	+	243	WP_152473095.1	hypothetical protein	-
FIU99_RS27165	68114..68617	+	504	WP_172974108.1	hypothetical protein	-
FIU99_RS27170	68617..69429	+	813	WP_152473073.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..95651	95651

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (2-91)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (5-84)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11262.71 Da
• Isoelectric Point: 5.6133

>T141745 WP_152473071.1 NZ_CP045358:c67346-67062 [Vibrio sp. THAF64]
MEFRDSYLEDFYENDVAHRKIPSNLENVLFRKLQMLDAATDYRDLLIPPNNQFEHLNGKLAGYSCIRVNRQYRLIFKFVD
GEVVDTYLDPHSYR

Nucleotide

• Length: 285 bp

>T141745 NZ_CP060075:3897068-3897409 [Escherichia coli]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGTGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTAGAAAAATACGAGCTGGTTCGTATCGACAGGAAGGGATTT
AGCTGGCAGGAACAATCTCCTTATCTCCGGGCTGCAGACATTCTGCGAGCGCGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 11976.87 Da
• Isoelectric Point: 9.7284

>AT141745 WP_152473070.1 NZ_CP045358:c66989-66654 [Vibrio sp. THAF64]
MRNTKRKPVTVGEMLKLEFLEPLAISNSDLASAIGVHRNTISNLINGKTALTSDLANKLAAALGNTPEFWLNIQHAVDVW
DSKNTFEVAKKAITPLVKIESSMLAGGYALA

Nucleotide

• Length: 336 bp

>AT141745 NZ_CP060075:3896730-3897047 [Escherichia coli]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGAGATTACACTGCGCCTGGGAGCCCGTCTGGTGCAGGAAGGCAACCGACT
GCATTATCTGGCTGACCGGGCCAGCATCACCGGCAAGTTCAGTGACATCGAATGCCGGAAGCTGGATGAAACATTCCCGC
ACTTTATCCGCCAGATGGAATCGATGCTGACCACCGGTGAACTCAGCCCCCACCATGCCCACTGCGTTACCCTGTACCAC
AACGATTTAACCTGCGAAGCCGACACCCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGCGTTAA