Detailed information of TA system TA141743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 208556..209385
• Replicon: plasmid pTHAF64_b
• Accession: NZ_CP045357
• Organism: Vibrio sp. THAF64

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: FIU99_RS25750
• Protein ID: WP_152472731.1
• Coordinates: 208822..209385 (+)
• Length: 188 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7Y3YYU7
• Locus tag: FIU99_RS25745
• Protein ID: WP_040122444.1
• Coordinates: 208556..208825 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FIU99_RS25690	203806..204138	+	333	WP_040122455.1	YnfA family protein	-
FIU99_RS25695	204238..204735	+	498	WP_152472725.1	DUF2867 domain-containing protein	-
FIU99_RS25700	204871..205248	+	378	WP_152472726.1	hypothetical protein	-
FIU99_RS25710	205378..205746	+	369	WP_152472727.1	hypothetical protein	-
FIU99_RS25715	205808..206305	+	498	WP_152472728.1	GNAT family N-acetyltransferase	-
FIU99_RS25725	206472..206948	+	477	WP_152472924.1	DUF1311 domain-containing protein	-
FIU99_RS25735	207395..207799	+	405	WP_152472729.1	NUDIX domain-containing protein	-
FIU99_RS25740	207927..208349	+	423	WP_152472730.1	GNAT family N-acetyltransferase	-
FIU99_RS25745	208556..208825	+	270	WP_040122444.1	DUF1778 domain-containing protein	Antitoxin
FIU99_RS25750	208822..209385	+	564	WP_152472731.1	GNAT family N-acetyltransferase	Toxin
FIU99_RS25760	210793..212886	+	2094	WP_152472732.1	TonB-dependent siderophore receptor	-
FIU99_RS25765	212951..213223	+	273	WP_152472733.1	hypothetical protein	-
FIU99_RS25770	213355..214323	+	969	WP_152472734.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	htpB	1..396761	396761

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (5-81)

Sequences

Toxin

Protein

• Length: 188 a.a.
• Molecular weight: 21099.61 Da
• Isoelectric Point: 9.3822

>T141743 WP_152472731.1 NZ_CP045357:208822-209385 [Vibrio sp. THAF64]
VSWGNEFVELSKSMHDRSSFDCGESELNTFIKTLAAKHMQAGISRTMVLPSSQPLLNQKYAICAFYSVAPSSISRETLPA
KLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKISLIRALKYLWEVNHHMRAYAIIVDCLTDSAQAFYTKFGFEVLCEHNG
RIRMFLPMKTVEQLFSLKATNITKDDV

Nucleotide

• Length: 564 bp

>T141743 NZ_CP060075:3725420-3725638 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 9839.34 Da
• Isoelectric Point: 4.5482

>AT141743 WP_040122444.1 NZ_CP045357:208556-208825 [Vibrio sp. THAF64]
MATARLDIRLDEEIKAKAEKASALLGLKSLTEYVVRLMDEDATQVIEQHESIVVKESVFDEFMMACDKAKAPNQALLDAA
KLTNESGIK

Nucleotide

• Length: 270 bp

>AT141743 NZ_CP060075:3725020-3725394 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A7Y3YYU7