Detailed information of TA system TA141482

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60125..60537
• Replicon: plasmid p65-4
• Accession: NZ_CP045211
• Organism: Escherichia coli strain 4

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: GC226_RS24975
• Protein ID: WP_223200913.1
• Coordinates: 60415..60537 (+)
• Length: 41 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	60125..60310 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GC226_RS24940 (55795)	55795..56322	+	528	WP_001562891.1	single-stranded DNA-binding protein	-
GC226_RS24945 (56380)	56380..56613	+	234	WP_000006003.1	DUF905 family protein	-
GC226_RS24950 (56674)	56674..58638	+	1965	WP_224068100.1	ParB/RepB/Spo0J family partition protein	-
GC226_RS24955 (58707)	58707..59141	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
GC226_RS24960 (59138)	59138..59857	+	720	WP_001276237.1	plasmid SOS inhibition protein A	-
GC226_RS24965 (59854)	59854..60168	+	315	WP_000872086.1	hypothetical protein	-
- (60125)	60125..60310	+	186	NuclAT_0	-	Antitoxin
- (60125)	60125..60310	+	186	NuclAT_0	-	Antitoxin
- (60125)	60125..60310	+	186	NuclAT_0	-	Antitoxin
- (60125)	60125..60310	+	186	NuclAT_0	-	Antitoxin
GC226_RS24970 (60318)	60318..60470	+	153	Protein_65	DUF5431 family protein	-
GC226_RS24975 (60415)	60415..60537	+	123	WP_223200913.1	Hok/Gef family protein	Toxin
GC226_RS24980 (60630)	60630..60794	+	165	WP_187415256.1	hypothetical protein	-
GC226_RS24985 (60863)	60863..61513	+	651	WP_228400228.1	IS66-like element accessory protein TnpA	-
GC226_RS24990 (61513)	61513..61860	+	348	WP_000624622.1	IS66 family insertion sequence element accessory protein TnpB	-
GC226_RS24995 (61880)	61880..63451	+	1572	WP_228400229.1	IS66 family transposase	-
GC226_RS25000 (63565)	63565..64026	+	462	WP_000760083.1	thermonuclease family protein	-
GC226_RS25005 (64129)	64129..64512	+	384	WP_228400230.1	hypothetical protein	-
- (64687)	64687..64743	-	57	NuclAT_1	-	-
- (64687)	64687..64743	-	57	NuclAT_1	-	-
- (64687)	64687..64743	-	57	NuclAT_1	-	-
- (64687)	64687..64743	-	57	NuclAT_1	-	-
GC226_RS25010 (64791)	64791..64940	+	150	WP_001336447.1	Hok/Gef family protein	-
GC226_RS25015 (65224)	65224..65481	+	258	WP_000084404.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..65782	65782

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (1-35)

Sequences

Toxin

Protein

• Length: 41 a.a.
• Molecular weight: 4538.38 Da
• Isoelectric Point: 8.2691

>T141482 WP_223200913.1 NZ_CP045211:60415-60537 [Escherichia coli]
IVCCTLLIFTLLTRNRLCEVRLKDGYREVTATMAYESGGK

Nucleotide

• Length: 123 bp

>T141482 NZ_CP059963:c359017-358712 [Pectobacterium brasiliense]
ATGCAATTCATTGTTTATCAATACAAACGCGCCAGTCACTACAAAATGTTTGTCGATGTGCAAAGCGATATTGTCGAGAC
ACCAAAGCGGCGTATGGTCATCCCGCTTATCGAAGCATATCACCTGTCTGAGAAAGTGAATAAAACATTGTTCCCTTTGA
TCCGCATCGACGGCGAAGATTATCGGCTGATGACAACTGAACTATCGAGCGTTCCCGTTGAGGTTATTGGTGAAGTGACT
GCGGATCTTGGCGACTACGCGGATGAGATTAAGGATGCTATTAATCTTATGTTTTGGGGGATTTGA

Antitoxin

Nucleotide

• Length: 186 bp

>AT141482 NZ_CP045211:60125-60310 [Escherichia coli]
CCCGTGAACTGGCTGAACGACCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGTGCGCAGGTATATGGGCTATGATGTG
CCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGCAGAAAGCCCCGT
AGTTAATTTTCATTAACCCACGAGGC

Structures

Antitoxin