Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1284831..1285470 Replicon chromosome
Accession NZ_CP044497
Organism Haemophilus influenzae strain PittGG

Toxin (Protein)


Gene name higB Uniprot ID A0A1B8PGE6
Locus tag F8B64_RS06450 Protein ID WP_012054677.1
Coordinates 1284831..1285136 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID H1LMA4
Locus tag F8B64_RS06455 Protein ID WP_009499989.1
Coordinates 1285147..1285470 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F8B64_RS06430 1280261..1282300 - 2040 WP_074039806.1 excinuclease ABC subunit B -
F8B64_RS06440 1282915..1283883 - 969 WP_074039804.1 zinc transporter permease subunit ZevB -
F8B64_RS06445 1283886..1284506 - 621 WP_074039803.1 zinc transporter binding subunit ZevA -
F8B64_RS06450 1284831..1285136 + 306 WP_012054677.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
F8B64_RS06455 1285147..1285470 + 324 WP_009499989.1 HigA family addiction module antidote protein Antitoxin
F8B64_RS06460 1285635..1287305 + 1671 WP_012054676.1 energy-dependent translational throttle protein EttA -
F8B64_RS06465 1287367..1287708 + 342 WP_005666735.1 SirB2 family protein -
F8B64_RS06470 1287713..1289557 + 1845 WP_012054674.1 tRNA(Met) cytidine acetyltransferase TmcA -
F8B64_RS09465 1289554..1289727 + 174 WP_012054673.1 DUF5363 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(7-97)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-67)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 12180.80 Da        Isoelectric Point: 8.3426

>T141021 WP_012054677.1 NZ_CP044497:1284831-1285136 [Haemophilus influenzae]
MFNLKREHFRDDYLYRFYQYGDAHSKIPSNLSKVLARKLDMISAAEDINDLRSPPANHLELLEPKENKIYSIRVNKQYRL
IFKYENNEVNNLYLDPHSYNL

Download         Length: 306 bp

>T141021 NZ_CP059711:c1083681-1083463 [Enterobacter hormaechei]
ATGTCTGATAAACCATTAACTAAAGTCGATTATTTGATGCGCCTGCGACGCTGTCAGTCAATTGACACGCTCGAACGTGT
TATTGAGAAAAATAAATACGAGCTGTCTGATAATGAACTGGCGGTATTTTATTCAGCTGCCGACCATCGTCTTGCTGAAC
TGACCATGAATAAACTCTATGACAAGATCCCCTCTTCGGTATGGAAATTTGTTCGTTAG

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12302.14 Da        Isoelectric Point: 7.4050

>AT141021 WP_009499989.1 NZ_CP044497:1285147-1285470 [Haemophilus influenzae]
MMTRKPTSVGEILQEEFLEPLSLKINDLAQILDVHRNTASNIVNNSSRITLEMAVKLAKAFDTTPEFWLNLQTRIDLWEL
EHNKRFQQSLANVKPALHRHGTSTFAM

Download         Length: 324 bp

>AT141021 NZ_CP059711:c1084084-1083710 [Enterobacter hormaechei]
ATGGACGAGTACTCGCCAAAAAGGCATGATATCGCGCAGTTGAAATTTCTCTGCGAATCCTTGTACCATGACTGCCTTGC
CAATCTTGAAGAAAGTAACCATGGCTGGGTGAACGATCCAACGTCTGCTATCAATTTACAGCTTAATGAGCTGATCGAGC
ATATCGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAAGATAATAAGTTGATAGAGCAAATTGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTACGGCATTAACGCACAGGATTTGCAGAAATGGCGTAAATCAGGAAACCGTCT
ATTTCGCTGCTTCACGAATGTCAGCAGAGCTAATCCGGTTAGTCTTTCCTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1B8PGE6

Antitoxin

Source ID Structure
AlphaFold DB H1LMA4

References