Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3821573..3822123 Replicon chromosome
Accession NZ_CP044443
Organism Escherichia coli strain HeN100

Toxin (Protein)


Gene name ccdB Uniprot ID D7Y6T6
Locus tag HeN100_RS20405 Protein ID WP_001160971.1
Coordinates 3821573..3821887 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag HeN100_RS20410 Protein ID WP_000125566.1
Coordinates 3821890..3822123 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HeN100_RS20380 (3817248) 3817248..3818534 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
HeN100_RS20385 (3818534) 3818534..3819523 + 990 WP_000241253.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
HeN100_RS20390 (3819520) 3819520..3820341 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
HeN100_RS20395 (3820344) 3820344..3820721 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
HeN100_RS20400 (3820728) 3820728..3821576 + 849 WP_000257189.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
HeN100_RS20405 (3821573) 3821573..3821887 - 315 WP_001160971.1 CcdB family protein Toxin
HeN100_RS20410 (3821890) 3821890..3822123 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
HeN100_RS20415 (3822209) 3822209..3822688 - 480 WP_001487721.1 type 3 dihydrofolate reductase -
HeN100_RS20420 (3822880) 3822880..3824742 - 1863 WP_072662688.1 glutathione-regulated potassium-efflux system protein KefC -
HeN100_RS20425 (3824735) 3824735..3825265 - 531 WP_000600735.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
HeN100_RS20430 (3825373) 3825373..3826704 - 1332 WP_001183198.1 MFS transporter -
HeN100_RS20435 (3826763) 3826763..3827050 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11741.75 Da        Isoelectric Point: 9.0851

>T140974 WP_001160971.1 NZ_CP044443:c3821887-3821573 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T140974 NZ_CP059586:2063735-2063986 [Xanthomonas oryzae pv. oryzae]
ATGCTACGGCTGCGTGAATTCCTGAGGCCGAAGAATCCCGATGCCGCGAGGCGGGCCGGCGAAGCGATTAGGCGCGGCGT
CCAGGCTCTCGGTGCCCACCCTCGAATGGGGCGGCTCATAGAAGATCTGTCTGAACAGTATCGGGAGTGGCTTATCGATT
TTGGGGATAGCGGCTATGTGGCTCGGTACCGCTTCGATGGGGATACCGTCACGATTTTGGCCGTGCGACACCAGAAGGAA
GCGGGGTACTGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT140974 WP_000125566.1 NZ_CP044443:c3822123-3821890 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT140974 NZ_CP059586:2063430-2063708 [Xanthomonas oryzae pv. oryzae]
ATGGTTACGGCAACTTCCATCAAGCTTGATGATGAATTGAAAGGGCGGGTTCAGCACTTGGCAGAGTCGCGCCGGCGTAC
CCCGCATTGGATCATGCGCGAAGCCATTGAGCAGTACGTCGAGCGCGAGGAAAAGCGCGAGGCGCTGAACCGGGACACGC
TCAAGGCATGGGATGAATTTCAGGCGACTGGCCTGCATGTGACCGCTGAGGAAGTGGACAAGTGGCTCGCGAGCTGGGGA
ACTGACGACGAACTGCCTCCGCCAGAATGCCACAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A241QVQ9

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References