Detailed information of TA system TA140844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 2647275..2647801
• Replicon: chromosome
• Accession: NZ_CP044403
• Organism: Escherichia coli strain NMBU-W10C18

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: F7O57_RS13045
• Protein ID: WP_000323025.1
• Coordinates: 2647275..2647562 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: F7O57_RS13050
• Protein ID: WP_000534858.1
• Coordinates: 2647562..2647801 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7O57_RS13000	2642359..2642817	-	459	WP_000526135.1	IS200/IS605 family transposase	-
F7O57_RS13005	2643268..2643483	-	216	WP_000066484.1	cold shock-like protein CspB	-
F7O57_RS13010	2643784..2643996	+	213	WP_000087756.1	cold shock-like protein CspF	-
F7O57_RS13015	2644051..2644140	+	90	WP_120795389.1	hypothetical protein	-
F7O57_RS13020	2644418..2645170	-	753	WP_001047135.1	antitermination protein	-
F7O57_RS13025	2645184..2646233	-	1050	WP_001265199.1	DUF968 domain-containing protein	-
F7O57_RS13030	2646235..2646513	-	279	WP_012304870.1	hypothetical protein	-
F7O57_RS13035	2646580..2646831	-	252	WP_000980994.1	hypothetical protein	-
F7O57_RS13040	2647048..2647203	-	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
F7O57_RS13045	2647275..2647562	-	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
F7O57_RS13050	2647562..2647801	-	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
F7O57_RS13055	2647826..2648131	+	306	WP_001326990.1	hypothetical protein	-
F7O57_RS13060	2648334..2648666	+	333	WP_001301033.1	protein FlxA	-
F7O57_RS13065	2649103..2650416	-	1314	WP_000589005.1	ISNCY family transposase	-
F7O57_RS13070	2650900..2651922	-	1023	WP_000915483.1	DUF4868 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2614384..2665734	51350
	-	flank	IS/Tn	-	-	2642359..2642817	458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T140844 WP_000323025.1 NZ_CP044403:c2647562-2647275 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T140844 NZ_CP059479:1170778-1171167 [Acinetobacter baumannii]
TTGATAAAAGAATTAAATTTTGAGCTGAAATATAGCCTTGTCTCGGTAATATTTCAGCTTTTTGTTGGTTTGGGTTTAGC
AATCCTGCTTTATCAATTATTAACACCGATATGGTGGCTATGTGCTGTCATTCTTCTATTTATTGGTTTTATTTTCTTTC
TAAAGCAAGCCCAAATATCTCAAATTGAATATTTAGATCAAAAATTATGGTCGGTTGCATATTTTTCGAAAAAAGAAATT
TACCGCGCGGAAATTACTAAAATAATTGATTATCAGTTATTTGTAGTTATTTATCTTGAAGAAACTCCTACGATCATAGC
TATTGTTTGGTTCGATCAGCTTCCTATTCAGCAGTGGAAAAGATTAAAAGTTTTAGAAAAGCTCTATTAA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT140844 WP_000534858.1 NZ_CP044403:c2647801-2647562 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT140844 NZ_CP059479:1170526-1170771 [Acinetobacter baumannii]
ATGACCTTGGAAGAACGTAAAGTAATTTATCGAGCACGCCGCGGCTTAAAAGAAATTGACGTCTATTTTGACCCGTATGT
AAAAAACTATTATTTAAAAGCTGATCCAGCAGAAAAAGCATTATTTGCAGAGTTAGTCGATCAAGAAGATCCGGATTTAT
TAGATTGGTTCATGGAAGTTTCTGAACCTCCACGTACTGAGTTACGTGAATTTATTTATAAACTAAAACAGTATGTGCAT
GGCTAA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8