Detailed information of TA system TA140625

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2501059..2501894
• Replicon: chromosome
• Accession: NZ_CP044313
• Organism: Escherichia coli strain RM11911

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: F7D04_RS12540
• Protein ID: WP_050938921.1
• Coordinates: 2501517..2501894 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0H0IVG5
• Locus tag: F7D04_RS12535
• Protein ID: WP_001339471.1
• Coordinates: 2501059..2501427 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D04_RS12505	2497895..2498350	+	456	WP_000581504.1	hypothetical protein	-
F7D04_RS12510	2498429..2498662	+	234	WP_001119729.1	DUF905 family protein	-
F7D04_RS12515	2498762..2499580	+	819	WP_001234620.1	DUF945 domain-containing protein	-
F7D04_RS12520	2499635..2500120	+	486	WP_050938918.1	antirestriction protein	-
F7D04_RS12525	2500136..2500612	+	477	WP_001186727.1	RadC family protein	-
F7D04_RS12530	2500675..2500896	+	222	WP_050938919.1	DUF987 domain-containing protein	-
F7D04_RS12535	2501059..2501427	+	369	WP_001339471.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
F7D04_RS12540	2501517..2501894	+	378	WP_050938921.1	TA system toxin CbtA family protein	Toxin
F7D04_RS12545	2501891..2502379	+	489	WP_000761721.1	hypothetical protein	-
F7D04_RS12550	2502391..2502588	+	198	WP_000839281.1	DUF957 domain-containing protein	-
F7D04_RS12555	2502685..2503257	+	573	WP_001290246.1	DUF4942 domain-containing protein	-
F7D04_RS12560	2503538..2504074	-	537	WP_000942795.1	GspM family type II secretion system protein YghD	-
F7D04_RS12565	2504076..2505254	-	1179	WP_000094974.1	type II secretion system protein GspL	-
F7D04_RS12570	2505251..2506228	-	978	WP_000633238.1	type II secretion system minor pseudopilin GspK	-
F7D04_RS12575	2506231..2506830	-	600	WP_064760790.1	type II secretion system minor pseudopilin GspJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	gspM / gspL / gspK / gspJ / gspI / gspH / gspG / gspF / gspE / gspE / gspD / gspC / rfaE	2457056..2641692	184636

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14209.18 Da
• Isoelectric Point: 7.7761

>T140625 WP_050938921.1 NZ_CP044313:2501517-2501894 [Escherichia coli]
MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYTLVRTDQPEF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYQEAKR

Nucleotide

• Length: 378 bp

>T140625 NZ_CP059325:c1947057-1946875 [Mannheimia haemolytica]
ATGAAATACAGCGAGTTCCTGAGGTACTTACTTGCTCAAGGTTGCGAAATTGAAAACCATAGGCGAGGCAGTCATCGAAA
GGTAACACTAAACGGAAAACAATCGGTTTTTCCTTATCACGGGAGCAAAGAGATTGGTACAGGTTTAGTAAATAAGATTA
AGAAAGACTTAGATCTTAAATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13517.22 Da
• Isoelectric Point: 6.4789

>AT140625 WP_001339471.1 NZ_CP044313:2501059-2501427 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLGSGGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT140625 NZ_CP059325:c1946836-1946417 [Mannheimia haemolytica]
ATGTTACGCTACCCAGTTGAAATTACCCCTGATGATAACGGCACATATCTTGTGACTTGCCCTGATATTCCTGAAATGGC
AAGCGTAGGTGAGGACTTAGAAGAAGCCCTATTAGAAGCACAAGATGGCTTAGCCACAGCCCTTGAATTCTATTTTGATG
ATAGACGAGAAATACCAATGCCAAGCCCAATTAAAGAGGGACAACATACGGTTAATCTTACCGTGCTGCAATCAATGAAA
GTATTTCTCTTAAATGAAATGATTAAACAAGGTGTACGCAAAGCAGAAATGGCAAGACGATTAGATGTACACTTACCACA
GATAGATCGCCTATTAGATTTTAATCATTCCACAAAAGTAGAATTTGTTGAGAAAGCCTATGGTAAGCTAAATCAACACT
TTACTATTCTCCCTCACTAA

Structures

Antitoxin

• AlphaFold DB: A0A0H0IVG5