Detailed information of TA system TA140619

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1273903..1274734
• Replicon: chromosome
• Accession: NZ_CP044313
• Organism: Escherichia coli strain RM11911

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: F7D04_RS06775
• Protein ID: WP_000854814.1
• Coordinates: 1274360..1274734 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A0H0EA76
• Locus tag: F7D04_RS06770
• Protein ID: WP_001285592.1
• Coordinates: 1273903..1274271 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F7D04_RS06740	1270807..1271262	+	456	WP_000581504.1	hypothetical protein	-
F7D04_RS06745	1271341..1271574	+	234	WP_001119724.1	DUF905 family protein	-
F7D04_RS06750	1271674..1272492	+	819	WP_047090156.1	DUF945 domain-containing protein	-
F7D04_RS06755	1272574..1273053	+	480	WP_000860086.1	antirestriction protein	-
F7D04_RS06760	1273069..1273545	+	477	WP_001186726.1	RadC family protein	-
F7D04_RS06765	1273608..1273829	+	222	WP_000692323.1	DUF987 domain-containing protein	-
F7D04_RS06770	1273903..1274271	+	369	WP_001285592.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
F7D04_RS06775	1274360..1274734	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
F7D04_RS06780	1274731..1274925	+	195	WP_000988600.1	hypothetical protein	-
F7D04_RS06785	1274938..1275051	+	114	WP_001161660.1	DUF957 domain-containing protein	-
F7D04_RS06790	1275340..1275468	-	129	Protein_1335	transposase domain-containing protein	-
F7D04_RS06795	1275540..1275722	+	183	WP_001016348.1	hypothetical protein	-
F7D04_RS06800	1275823..1276152	-	330	WP_000450409.1	DUF496 family protein	-
F7D04_RS06805	1276324..1276842	-	519	Protein_1338	hypothetical protein	-
F7D04_RS06810	1276937..1277917	-	981	WP_000399648.1	IS110-like element IS621 family transposase	-
F7D04_RS06815	1277981..1278661	-	681	WP_001200880.1	FUSC family protein	-
F7D04_RS06820	1278859..1279332	-	474	WP_001105415.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1276937..1277917	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T140619 WP_000854814.1 NZ_CP044313:1274360-1274734 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T140619 NZ_CP059325:11849-12073 [Mannheimia haemolytica]
ATGGTAGTAAAAATCCTAAAGCGGAAATCTCAAAAGACACTACAACAAATCTACACCCAGCCTATTTCTGCCAATATTAA
ATGGACAAATATAGAAGCTCTTTTTCTAGAGTTAGGAGCAGAAATAGAAGAACGAGAAGGGAGCAGAATTGCAGTTGTTT
TATTTCAACAGGTCAGAGTATTTCATCGCCCACACCCGAATCCTGATACAGATAAGGGGCGGTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13636.51 Da
• Isoelectric Point: 6.6249

>AT140619 WP_001285592.1 NZ_CP044313:1273903-1274271 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT140619 NZ_CP059325:12116-12445 [Mannheimia haemolytica]
ATGCGTAATATTATGGATATTAATGGTTATAAAGCTGTCATTGCTTACGATCCCGAAACAGAATTATTTCGAGGTGAATT
TATCGGATTAAATGGTGGAGCCGATTTTTATGCCGATAATGTCATTCAATTAAAAAAAGAAGGTGAACTAAGTTTGAATA
TCTTTTTAGAACTTTGTAGAGAAAAAGGTATTGAGCCGTTCAAACAATATTCCGGAAAGTTTAATGTTCGCCTTTCGCCT
GAATTACATAAAGCTGCGGTAATTGCAGCAACTGCTGAAAATTTAAGCTTAAATGAATGGATTAACCAAACGTTAGAAAA
GAGCGTGTAG

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A0H0EA76