Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 4253682..4254468 Replicon chromosome
Accession NZ_CP044218
Organism Mesorhizobium terrae strain NIBRBAC000500504

Toxin (Protein)


Gene name tacT Uniprot ID A0A1C2DDA7
Locus tag FZF13_RS21685 Protein ID WP_024925070.1
Coordinates 4253971..4254468 (+) Length 166 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A1C2DD80
Locus tag FZF13_RS21680 Protein ID WP_024925069.1
Coordinates 4253682..4253966 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FZF13_RS21655 4248907..4249425 + 519 WP_024925064.1 DUF2840 domain-containing protein -
FZF13_RS21660 4249422..4249967 + 546 WP_024925065.1 S26 family signal peptidase -
FZF13_RS21665 4250004..4250339 + 336 WP_024925066.1 DUF736 domain-containing protein -
FZF13_RS21670 4250345..4251268 + 924 WP_024925067.1 lytic transglycosylase domain-containing protein -
FZF13_RS21675 4251561..4253609 + 2049 WP_024925068.1 relaxase/mobilization nuclease and DUF3363 domain-containing protein -
FZF13_RS21680 4253682..4253966 + 285 WP_024925069.1 DUF1778 domain-containing protein Antitoxin
FZF13_RS21685 4253971..4254468 + 498 WP_024925070.1 GNAT family N-acetyltransferase Toxin
FZF13_RS21690 4254465..4254749 - 285 WP_024925071.1 helix-turn-helix domain-containing protein -
FZF13_RS21695 4254857..4255171 + 315 WP_024925072.1 DUF2285 domain-containing protein -
FZF13_RS21700 4255293..4257281 + 1989 WP_024925073.1 conjugal transfer protein TraG -
FZF13_RS21705 4257291..4257752 + 462 WP_024925074.1 ribbon-helix-helix domain-containing protein -
FZF13_RS21710 4257895..4258911 + 1017 WP_024925075.1 P-type conjugative transfer ATPase TrbB -
FZF13_RS21715 4258929..4259255 + 327 WP_036253476.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - - 4219796..4263664 43868


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(35-142)

Antitoxin

Vibrio phage ICP1, Orf50

(9-84)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18183.91 Da        Isoelectric Point: 8.0738

>T140461 WP_024925070.1 NZ_CP044218:4253971-4254468 [Mesorhizobium terrae]
VALRIEKLRREHRVEGFDCGKEPLNRFLIRFALQSQLSNSAQTYLALSDDEVVGYYTLTYGDVAYDDAPGRLQKGVARHP
VPLMILARLAVASAWSGKGIGSGLLKDALGRTLAAAEIAGLRAFAVHAKDEEARAFYEHFDFIASPSDPMHLFVLLKDVR
ALIGR

Download         Length: 498 bp

>T140461 NZ_CP059280:138355-138660 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10610.11 Da        Isoelectric Point: 7.2069

>AT140461 WP_024925069.1 NZ_CP044218:4253682-4253966 [Mesorhizobium terrae]
MANATRSEKLDLRLTPEAKRVLNAAAQAERRSVSEFVLESALIRAQETLPDRQHFGLDAERWQAFLAALDAPPRPLPRLK
RLLNEPGLFEHPGE

Download         Length: 285 bp

>AT140461 NZ_CP059280:138135-138353 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1C2DDA7

Antitoxin

Source ID Structure
AlphaFold DB A0A1C2DD80

References