140418

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 3509164..3509891 Replicon chromosome
Accession NZ_CP044158
Organism Shigella flexneri strain AR-0423

Toxin (Protein)


Gene name higB Uniprot ID U9ZMR4
Locus tag F4V35_RS18165 Protein ID WP_000550189.1
Coordinates 3509577..3509891 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag F4V35_RS18160 Protein ID WP_000560266.1
Coordinates 3509164..3509580 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V35_RS18150 (3504524) 3504524..3506875 + 2352 WP_000695506.1 alpha-glucosidase -
F4V35_RS18155 (3507101) 3507101..3509119 + 2019 WP_000121487.1 NADPH-dependent 2,4-dienoyl-CoA reductase -
F4V35_RS18160 (3509164) 3509164..3509580 - 417 WP_000560266.1 type II toxin-antitoxin system antitoxin HigA Antitoxin
F4V35_RS18165 (3509577) 3509577..3509891 - 315 WP_000550189.1 type II toxin-antitoxin system toxin HigB Toxin
F4V35_RS18170 (3510176) 3510176..3511312 - 1137 WP_000018656.1 23S rRNA (guanine(1835)-N(2))-methyltransferase RlmG -
F4V35_RS18175 (3511397) 3511397..3511900 + 504 WP_005050988.1 M48 family metallopeptidase -
F4V35_RS18180 (3511977) 3511977..3512669 + 693 WP_000942538.1 vancomycin high temperature exclusion protein -
F4V35_RS18185 (3512748) 3512748..3513746 + 999 WP_005050984.1 Gfo/Idh/MocA family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-135)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12103.10 Da        Isoelectric Point: 10.0409

>T140418 WP_000550189.1 NZ_CP044158:c3509891-3509577 [Shigella flexneri]
MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAVFPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKC
YIREVMTHKEYDFFTAVHRTKGKK

Download         Length: 315 bp

>T140418 NZ_CP059156:c2259253-2259149 [Staphylococcus aureus]
TTGTTACTCCTAGAAAGGACTAGCATGTCTGATTTTGAAATGCTGATGATTGTATTAACAATCATTGGTTTAGTATTGAT
TAGTACTCAAGACCATAAAAAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 14995.42 Da        Isoelectric Point: 4.4547

>AT140418 WP_000560266.1 NZ_CP044158:c3509580-3509164 [Shigella flexneri]
MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLNDPENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGG
IAVIRTLMDQYGLTLSDLPEIGSKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID

Download         Length: 417 bp

>AT140418 NZ_CP059156:2259056-2259094 [Staphylococcus aureus]
AACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGAC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6KMQ
PDB 5IFG
PDB 5YCL
PDB 6KML

Antitoxin

Source ID Structure

References