Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1362557..1363352 Replicon chromosome
Accession NZ_CP044158
Organism Shigella flexneri strain AR-0423

Toxin (Protein)


Gene name yeeV Uniprot ID Q93EZ7
Locus tag F4V35_RS06910 Protein ID WP_000854917.1
Coordinates 1362978..1363352 (+) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D2ACA1
Locus tag F4V35_RS06905 Protein ID WP_001280958.1
Coordinates 1362557..1362931 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V35_RS06860 (1357971) 1357971..1358387 + 417 WP_000275180.1 AraC family transcriptional regulator -
F4V35_RS06865 (1358446) 1358446..1359143 + 698 WP_095033700.1 IS1-like element IS1B family transposase -
F4V35_RS25630 (1359326) 1359326..1359589 + 264 WP_000466628.1 DNA polymerase III subunit gamma/tau -
F4V35_RS06875 (1359709) 1359709..1359882 + 174 WP_000088744.1 DUF905 family protein -
F4V35_RS06880 (1359982) 1359982..1360803 + 822 WP_001234584.1 DUF932 domain-containing protein -
F4V35_RS06890 (1361145) 1361145..1361618 + 474 WP_005011597.1 antirestriction protein -
F4V35_RS06895 (1361634) 1361634..1362110 + 477 WP_005011624.1 RadC family protein -
F4V35_RS06900 (1362173) 1362173..1362394 + 222 WP_001220314.1 DUF987 domain-containing protein -
F4V35_RS06905 (1362557) 1362557..1362931 + 375 WP_001280958.1 type IV toxin-antitoxin system YeeU family antitoxin Antitoxin
F4V35_RS06910 (1362978) 1362978..1363352 + 375 WP_000854917.1 TA system toxin CbtA family protein Toxin
F4V35_RS06915 (1363349) 1363349..1363840 + 492 WP_000976828.1 DUF5983 family protein -
F4V35_RS06920 (1363852) 1363852..1364049 + 198 WP_000839282.1 DUF957 domain-containing protein -
F4V35_RS06925 (1364134) 1364134..1364694 + 561 Protein_1331 DUF4942 domain-containing protein -
F4V35_RS06930 (1364766) 1364766..1365770 + 1005 WP_001179707.1 IS110-like element ISSfl8 family transposase -
F4V35_RS06935 (1366052) 1366052..1366333 + 282 Protein_1333 DUF4942 domain-containing protein -
F4V35_RS06945 (1366805) 1366805..1367743 + 939 Protein_1334 glyoxylate/hydroxypyruvate reductase GhrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 1364766..1365770 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 14145.22 Da        Isoelectric Point: 7.7761

>T140400 WP_000854917.1 NZ_CP044158:1362978-1363352 [Shigella flexneri]
MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCPRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T140400 NZ_CP059155:c1989831-1989679 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 13793.62 Da        Isoelectric Point: 6.4651

>AT140400 WP_001280958.1 NZ_CP044158:1362557-1362931 [Shigella flexneri]
VSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRYQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS

Download         Length: 375 bp

>AT140400 NZ_CP059155:c1990455-1989856 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q93EZ7

Antitoxin

Source ID Structure
AlphaFold DB A0A0K4PAV2

References