Detailed information of TA system TA140385

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 740..1166
• Replicon: plasmid pAR-0424-1
• Accession: NZ_CP044156
• Organism: Shigella flexneri strain AR-0424

Toxin (Protein)

• Gene name: hok
• Uniprot ID: -
• Locus tag: F4V36_RS23415
• Protein ID: WP_001372321.1
• Coordinates: 740..865 (-)
• Length: 42 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	942..1166 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V36_RS23410 (125)	125..421	+	297	Protein_0	hypothetical protein	-
F4V36_RS23415 (740)	740..865	-	126	WP_001372321.1	type I toxin-antitoxin system Hok family toxin	Toxin
F4V36_RS26330 (807)	807..956	-	150	Protein_2	plasmid maintenance protein Mok	-
F4V36_RS23420 (978)	978..1157	+	180	WP_001334662.1	hypothetical protein	-
- (942)	942..1166	-	225	NuclAT_0	-	Antitoxin
- (942)	942..1166	-	225	NuclAT_0	-	Antitoxin
- (942)	942..1166	-	225	NuclAT_0	-	Antitoxin
- (942)	942..1166	-	225	NuclAT_0	-	Antitoxin
F4V36_RS23425 (1135)	1135..1897	-	763	Protein_4	plasmid SOS inhibition protein A	-
F4V36_RS23430 (1894)	1894..2328	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
F4V36_RS23435 (2382)	2382..4340	-	1959	WP_112324844.1	ParB/RepB/Spo0J family partition protein	-
F4V36_RS23440 (4404)	4404..4637	-	234	WP_000005971.1	DUF905 domain-containing protein	-
F4V36_RS23445 (4693)	4693..5214	-	522	WP_033807850.1	single-stranded DNA-binding protein	-
F4V36_RS26335 (5513)	5513..5956	+	444	Protein_9	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	erm(B) / mph(A) / sul1 / qacE / aadA5 / blaTEM-1B	-	1..79831	79831

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (2-37)

Sequences

Toxin

Protein

• Length: 42 a.a.
• Molecular weight: 4780.69 Da
• Isoelectric Point: 8.5110

>T140385 WP_001372321.1 NZ_CP044156:c865-740 [Shigella flexneri]
VLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 126 bp

>T140385 NZ_CP059130:4164702-4165079 [Escherichia coli]
ATGAAAACATTACCCGACACACACGTACGGGCGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCAACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Nucleotide

• Length: 225 bp

>AT140385 NZ_CP044156:c1166-942 [Shigella flexneri]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Antitoxin