Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 132602..133240 Replicon chromosome
Accession NZ_CP044148
Organism Escherichia coli O157 strain AR-0427

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag F4V39_RS00595 Protein ID WP_000813794.1
Coordinates 132602..132778 (+) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag F4V39_RS00600 Protein ID WP_001270286.1
Coordinates 132824..133240 (+) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V39_RS00575 128223..129396 - 1174 Protein_116 BenE family transporter YdcO -
F4V39_RS00580 129488..130024 + 537 WP_000429145.1 DNA-binding transcriptional regulator SutR -
F4V39_RS00585 130097..132058 + 1962 WP_001303492.1 23S rRNA 5-hydroxycytidine C2501 synthase -
F4V39_RS00590 132150..132380 - 231 WP_000494244.1 YncJ family protein -
F4V39_RS00595 132602..132778 + 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
F4V39_RS00600 132824..133240 + 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
F4V39_RS00605 133319..134725 + 1407 WP_000760654.1 PLP-dependent aminotransferase family protein -
F4V39_RS00610 134970..136115 + 1146 WP_000047432.1 ABC transporter substrate-binding protein -
F4V39_RS00615 136133..137146 + 1014 WP_000220402.1 ABC transporter ATP-binding protein -
F4V39_RS00620 137147..138088 + 942 WP_001251315.1 ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T140274 WP_000813794.1 NZ_CP044148:132602-132778 [Escherichia coli O157]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T140274 NZ_CP059068:3874465-3874875 [Rhodoferax sp. AJA081-3]
ATGATCCTGCTCGATACCGTGGTTCTGTCGGAGTTGCGCAAGACAAAGCCGAATGCCAATGTGACAAACTATCTCAAAGC
GCAGGCCGAAGGCACCACATTCCTAAGTGCCTTGTCGATTGGTGAAATTGAAGCTGGCATTGAACGTCAGCGCGGTCACA
ATCCCGCGTTTGCGGACGAACTGGCGCAATGGTTGGTGGCATTGGAGCTGCAGTTCTCACAGTACATATTGCCGGTGACC
CCTGCGATCGCCAAACTATGGGGGCGGCTGTGTGTGCAGACCGGTAACAAGGGGATAGACAACCTGATCGCTGCAACCGC
GCTATGCCACAACCTCAGCGTTGTCACGCGCAATACCAAACACTTTGAACCTACGGGTGTTCGGGTGTTGAATCCGTTCG
ACGCGCAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT140274 WP_001270286.1 NZ_CP044148:132824-133240 [Escherichia coli O157]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT140274 NZ_CP059068:3874145-3874468 [Rhodoferax sp. AJA081-3]
ATGCAAACGCCTCCTACACTATCGCCAGCAAATGCTTGGCGACTGCAAGACGCCAAGGCGCAATTCAGCGAGTTGGTGGA
CAACGCCATGCGTGGCGTGCCCCAGCACGTCACGCGGCGTGGAAAACAAGCCGTGGTGGTGCTGTCGGAGGCAGATTTCG
AGGCACTGCAGAGAAATGCGGCCGCCAAGCAACCCAGGGGCCTGATCGAACACTTGCTGGCCATTCCAAAGACGCCCGTG
CCTTATCAGTCCAAAGAGCGCACGGTAGCGGACGCAGAGTTCCCCCGCCTTGACCTGCAGCCACGGGAGATTGACTTTTC
ATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References