Detailed information of TA system TA140222

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3242948..3243747
• Replicon: chromosome
• Accession: NZ_CP044143
• Organism: Escherichia coli O157 strain AR-0429

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: F4V41_RS17095
• Protein ID: WP_000347273.1
• Coordinates: 3242948..3243412 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: C3ST72
• Locus tag: F4V41_RS17100
• Protein ID: WP_001302819.1
• Coordinates: 3243412..3243747 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V41_RS17065	3237949..3238383	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
F4V41_RS17070	3238401..3239279	-	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
F4V41_RS17075	3239269..3240048	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
F4V41_RS17080	3240059..3240532	-	474	WP_001301475.1	PTS N-acetylgalactosamine transporter subunit IIB	-
F4V41_RS17085	3240555..3241835	-	1281	WP_000681927.1	tagatose-bisphosphate aldolase subunit KbaZ	-
F4V41_RS17090	3242084..3242893	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
F4V41_RS17095	3242948..3243412	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
F4V41_RS17100	3243412..3243747	-	336	WP_001302819.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
F4V41_RS17105	3243896..3245467	-	1572	WP_001273776.1	galactarate dehydratase	-
F4V41_RS17110	3245842..3247176	+	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
F4V41_RS17115	3247192..3247962	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T140222 WP_000347273.1 NZ_CP044143:c3243412-3242948 [Escherichia coli O157]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T140222 NZ_CP059043:c2521973-2521722 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12388.97 Da
• Isoelectric Point: 4.8616

>AT140222 WP_001302819.1 NZ_CP044143:c3243747-3243412 [Escherichia coli O157]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT140222 NZ_CP059043:c2521725-2521471 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• AlphaFold DB: V0SSH7