Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 2942339..2943139 Replicon chromosome
Accession NZ_CP044143
Organism Escherichia coli O157 strain AR-0429

Toxin (Protein)


Gene name ataT Uniprot ID A0A891SNC1
Locus tag F4V41_RS15530 Protein ID WP_000342448.1
Coordinates 2942612..2943139 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag F4V41_RS15525 Protein ID WP_001277108.1
Coordinates 2942339..2942605 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F4V41_RS15505 2937997..2938665 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
F4V41_RS15510 2938658..2939716 + 1059 WP_001042018.1 permease-like cell division protein FtsX -
F4V41_RS15515 2939961..2940815 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
F4V41_RS15520 2941086..2942189 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
F4V41_RS15525 2942339..2942605 + 267 WP_001277108.1 DUF1778 domain-containing protein Antitoxin
F4V41_RS15530 2942612..2943139 + 528 WP_000342448.1 GNAT family N-acetyltransferase Toxin
F4V41_RS15535 2943136..2943519 - 384 WP_000778769.1 aspartate 1-decarboxylase autocleavage activator PanM -
F4V41_RS15540 2943943..2945052 + 1110 WP_001301528.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
F4V41_RS15545 2945100..2946026 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
F4V41_RS15550 2946023..2947300 + 1278 WP_000803797.1 branched chain amino acid ABC transporter permease LivM -
F4V41_RS15555 2947297..2948064 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19692.68 Da        Isoelectric Point: 7.3232

>T140219 WP_000342448.1 NZ_CP044143:2942612-2943139 [Escherichia coli O157]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T140219 NZ_CP059043:c2356243-2356140 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT140219 WP_001277108.1 NZ_CP044143:2942339-2942605 [Escherichia coli O157]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT140219 NZ_CP059043:2356102-2356373 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6GTS

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References