Detailed information of TA system TA140216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 1994223..1994818
• Replicon: chromosome
• Accession: NZ_CP044143
• Organism: Escherichia coli O157 strain AR-0429

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: U9Y4M4
• Locus tag: F4V41_RS11015
• Protein ID: WP_000239579.1
• Coordinates: 1994223..1994573 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: Q8XCF3
• Locus tag: F4V41_RS11020
• Protein ID: WP_001223210.1
• Coordinates: 1994567..1994818 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
F4V41_RS10995	1989669..1990691	-	1023	WP_001301928.1	ABC transporter permease	-
F4V41_RS11000	1990705..1992207	-	1503	WP_000205806.1	sugar ABC transporter ATP-binding protein	-
F4V41_RS11005	1992347..1993303	-	957	WP_000265942.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
F4V41_RS11010	1993613..1994143	+	531	WP_000055075.1	inorganic diphosphatase	-
F4V41_RS11015	1994223..1994573	-	351	WP_000239579.1	endoribonuclease toxin ChpB	Toxin
F4V41_RS11020	1994567..1994818	-	252	WP_001223210.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
F4V41_RS11025	1995030..1995371	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
F4V41_RS11030	1995374..1999153	-	3780	WP_000060930.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12511.45 Da
• Isoelectric Point: 5.6219

>T140216 WP_000239579.1 NZ_CP044143:c1994573-1994223 [Escherichia coli O157]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T140216 NZ_CP059043:1942322-1942498 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9299.64 Da
• Isoelectric Point: 4.4201

>AT140216 WP_001223210.1 NZ_CP044143:c1994818-1994567 [Escherichia coli O157]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEVQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT140216 NZ_CP059043:1942544-1942960 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y8A8