Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 484905..485542 Replicon chromosome
Accession NZ_CP044133
Organism Bacillus velezensis strain FJAT-46737

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag F3129_RS02490 Protein ID WP_003156187.1
Coordinates 485192..485542 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag F3129_RS02485 Protein ID WP_003156188.1
Coordinates 484905..485186 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
F3129_RS02465 481270..481869 - 600 WP_020955355.1 rhomboid family intramembrane serine protease -
F3129_RS02470 481962..482327 + 366 WP_150941004.1 holo-ACP synthase -
F3129_RS02475 482492..483499 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
F3129_RS02480 483616..484785 + 1170 WP_150941005.1 alanine racemase -
F3129_RS02485 484905..485186 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
F3129_RS02490 485192..485542 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
F3129_RS02495 485660..486481 + 822 WP_015416874.1 STAS domain-containing protein -
F3129_RS02500 486486..486851 + 366 WP_003156180.1 RsbT antagonist protein RsbS -
F3129_RS02505 486854..487255 + 402 WP_003156178.1 anti-sigma regulatory factor -
F3129_RS02510 487267..488274 + 1008 WP_150941006.1 PP2C family protein-serine/threonine phosphatase -
F3129_RS02515 488338..488667 + 330 WP_033575044.1 anti-sigma factor antagonist -
F3129_RS02520 488664..489146 + 483 WP_007609591.1 anti-sigma B factor RsbW -
F3129_RS02525 489112..489900 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
F3129_RS02530 489900..490502 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T140156 WP_003156187.1 NZ_CP044133:485192-485542 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T140156 NZ_CP058971:c1713858-1713604 [Micrococcus luteus]
GTGCGCCTCGTGTGGGACCGCTCGGCCTGGGAGGACTACACGCACTGGCAGACCACGGACCGCAAGGTCCTCAAGCGGAT
CAACACCCTCATCGACGCCGCCCTGCGTGATCCCTTCGACGGGATCGGCAAGCCCGAGCAGCTGAAGTACGGCGCGCCCG
GGGCATGGTCCCGACGCATCACGGAGGAGCACAGGCTCGTCTACCTCGTCGACGGCGAGGACCTGATCATCCTCCAGGCG
AGGTATCACTACTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT140156 WP_003156188.1 NZ_CP044133:484905-485186 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT140156 NZ_CP058971:c1714119-1713859 [Micrococcus luteus]
ATGTCCATCAGCGCCAGTGAAGCCCGCAAGACCCTGTTCCCGCTCATCGAGCGAGTGAATGCGGACCGCGACGCCGTAGA
GATCGTCTCCCGCAAGGGGAATGCCGTCCTCATGCCCGCGGACGAGTACGCCGCCTGGCAGGAGACCGCCTACCTGTTCC
GCTCCCCGGCCAACGCCCGCCGGCTGCTGGACGCCTACGAGCGCGCCCGCGCAGGGAGCGTCGAGGAGCACGCTCTGGAC
CGCATGGACGGGGACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References