Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2261996..2262915 Replicon chromosome
Accession NZ_CP043996
Organism Mycobacterium tuberculosis strain 1-0072P6C4

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag FPJ52_RS10530 Protein ID WP_003900449.1
Coordinates 2262310..2262915 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag FPJ52_RS10525 Protein ID WP_003410124.1
Coordinates 2261996..2262301 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
FPJ52_RS10490 2257259..2257459 + 201 Protein_2073 hypothetical protein -
FPJ52_RS10500 2258815..2259192 + 378 Protein_2075 hypothetical protein -
FPJ52_RS10505 2259189..2260229 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
FPJ52_RS10510 2260471..2261190 + 720 WP_003410108.1 DUF433 domain-containing protein -
FPJ52_RS10515 2261180..2261596 + 417 WP_003410114.1 hypothetical protein -
FPJ52_RS10520 2261612..2261911 - 300 WP_003410120.1 hypothetical protein -
FPJ52_RS10525 2261996..2262301 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
FPJ52_RS10530 2262310..2262915 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
FPJ52_RS10535 2263061..2264322 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -
FPJ52_RS10540 2264349..2264657 - 309 WP_192948127.1 hypothetical protein -
FPJ52_RS10545 2264817..2265494 - 678 WP_003905725.1 hypothetical protein -
FPJ52_RS10550 2265446..2265682 - 237 WP_003410133.1 hypothetical protein -
FPJ52_RS10555 2265889..2266785 - 897 WP_003410136.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2257503..2273258 15755
- inside IScluster/Tn - - 2257503..2264322 6819


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T139783 WP_003900449.1 NZ_CP043996:c2262915-2262310 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T139783 NZ_CP058826:3286935-3287153 [Shigella flexneri]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT139783 WP_003410124.1 NZ_CP043996:c2262301-2261996 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT139783 NZ_CP058826:3286535-3286909 [Shigella flexneri]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References