Detailed information of TA system TA139748

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 361831..362402
• Replicon: chromosome
• Accession: NZ_CP043996
• Organism: Mycobacterium tuberculosis strain 1-0072P6C4

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: FPJ52_RS01565
• Protein ID: WP_003401560.1
• Coordinates: 361831..362133 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: FPJ52_RS01570
• Protein ID: WP_003401563.1
• Coordinates: 362181..362402 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
FPJ52_RS01545	357300..358103	-	804	WP_003401540.1	hypothetical protein	-
FPJ52_RS01550	358113..359510	-	1398	WP_003401544.1	sulfatase	-
FPJ52_RS01555	359689..361464	+	1776	WP_010886074.1	PE family protein	-
FPJ52_RS01560	361607..361834	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
FPJ52_RS01565	361831..362133	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
FPJ52_RS01570	362181..362402	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
FPJ52_RS01575	362399..362824	+	426	WP_003401566.1	PIN domain nuclease	-
FPJ52_RS01580	362960..363592	+	633	WP_003905275.1	TetR/AcrR family transcriptional regulator	-
FPJ52_RS01585	363589..364497	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T139748 WP_003401560.1 NZ_CP043996:361831-362133 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T139748 NZ_CP043996:361831-362133 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT139748 WP_003401563.1 NZ_CP043996:362181-362402 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT139748 NZ_CP058796:c188573-188517 [Shigella flexneri]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48